A New Model for in Vitro Corneal Epithelial Wound Healing Study

角膜表皮傷口癒合研究的一個新模式

林昌平(Chang-Ping Lin) ； 彭可(Matthias Boehnke)

《The Kaohsiung Journal of Medical Sciences》 13卷8期 (1997/08) Pp. 475-479

https://doi.org/10.6452/KJMS.199708.0475

摘要

本實驗之目的在建立一個無需照相記錄及計算傷口面積之活體外角膜表皮癒合模式。以準分子雷射在28隻豬眼角膜上切削表皮傷口，包括直徑2.omm ,8眼； 1.5mm, 12眼；所有傷口深度皆為 70μm。於切削後，將眼球置入培養箱中，於玻璃體中灌注TC-199 培養液，每組 8 眼以 TC-199 點眼，觀察其表皮癒合之速度。另外1.5mm , 4 眼則點 0.02 % Benzalkonium Chloride 。接受 TC-199 點眼之傷口平均癒合時間是： 2.0mm 組 31.75±6.18小時，l.8mm組28.75±3.85小時，1.5mm 組 24.50±1.77小時。以 Benzalkonium Chlo-ride 點眼之傷口經36小時仍未癒合。另外12個未經準分子雷射處理之豬眼，以相同條件培養以觀察眼壓之變化，於24小時後其眼壓為15.9至20.6mmHg，平均19mmHg。
本研究顯示於利用小傷口角膜表皮之癒合模式，可以約在24小時測知藥物對於角膜表皮傷口癒合之影響。

並列摘要

A new model for an epithelial wound healing study in pig eyes has been developed in which the excimer laser was used for ablation to create an epithelial wound with precise depth and size. The purpose of this study was to set up an in vitro epithelial wound healing model without photography and defect area measurement.
In the study twenty-eight pig eyes received phototherapeutic keratectomy (PTK) with the following diameters: eight at 2.0mm, eight at 1.8mm, and twelve at 1.5mm. All the wounds were 70gm in depth. Following PTK, the eyeballs were placed in an incubator, and perfused with TC-199 media by vitreous puncture. TC-199 was then used to rinse eight eyeballs from each group. The remaining four eyes from the 1.5mm group were rinsed with 0.02% Benzalkonium Chloride on an epithelial wound. For the eyes rinsed with TC-199 the average healing time for the 2.0mm group was 31.75 ± 6.18 hours; the 1.8mm group was 28.75 ± 3.85 hours, and the 1.5mm group was 24.5 ±1.77 hours. The corneas rinsed with Benzalkonium Chloride did not heal after 36 hour’s observation. Twelve eyeballs without PTK were perfused under the same criteria to evaluate the change of lOP. Following twenty-four hours the lOP range was 15.9 to 20.6mmllg with an average of 19.OmmHg.
This study revealed that the healing time decreased as the wound diameter became smaller and the healing was retarded by the drug effect. With this model, the drug influences on corneal epithelial wound healing can be evaluated and the result is revealed in approximately twenty-four hours.

並列關鍵字

corneal epithelium ； photokeratectomy ； wound healing