Influences of Hydrogel Contact Lens Care Solutions on Corneal Epithelial Wound Healing

軟式隱形眼鏡保養藥水對於豬角膜表皮傷口癒合之影響

林昌平(Chang-Ping Lin) ； 陳征宇(Jen-Yie Chen) ； 彭可(Matthias Boehnke)

《The Kaohsiung Journal of Medical Sciences》 14卷10期 (1998/10) Pp. 639-643

https://doi.org/10.6452/KJMS.199810.0639

摘要

隱形眼鏡葯水必須具備效率、安全及方便三大要素。葯水中的殺菌劑可殺死微生物，但也會對眼球表面造成傷害。多功能葯水強調其方便性，因此鏡片取出後，不用生理食鹽水沖洗，而直接戴到眼睛上。大量的葯水含在鏡片表面或裡面，直接接觸到眼睛，於軟式隱形眼鏡由於其高含水量，此種情形更加明顯。雙氧水會損傷眼球表面，因此需先中和後，鏡片才能配戴，中和不完全殘留之雙氧水，也可能傷害到眼球表面。我們以豬眼睛為材料，在體外測試這兩類葯水對於角膜表皮傷口癒合之影響。
雙氧水類的葯水包括AOSEPT、Oxysept和Contopharma-Peroxide-System。先依照祓的方法中和後，再滴於角膜傷口上。多功能葯水包括Opti-free、Hexidin和Bausch & Lomb則直接滴於角膜傷口上。豬眼球由屠宰場收集後，置於冰箱中帶回，先清除結締組織及結膜後，浸入PVP-iodine和Gentamycin中消毒。我們以準分子雷射於角膜表皮正中央切削一個直徑1.5mm，深度70µm之傷口，然後以一灌注系統灌注TC-199培義液，維持眼球於培養箱中，保持角膜朝正上方。於裝置完成後，立刻以TC-199培養液潤角膜表面，然後分別於1、3、5小時後，點實驗葯水或BSS共三次於傷口上。於24小時後傷口以螢光試劑染色，以藍色燈光檢查其傷口之大小，依其癒合程度予0至3分，0分為完全癒合。所得之分數以Mann-Whitney rank-sum test檢算。統計上所有隱形眼鏡葯水和BSS比較，均無延遲傷口癒合之現象，顯示這些隱形眼鏡葯水對於豬眼球角膜表皮於短時間內並不具毒性。

並列摘要

We studied the influences of hydrogel contact lens care solutions on corneal epithelial wound healing in vitro. The corneal epithelial wounds, 1.5MM in diameter, 70µm in depth were created on the pig’s eyeballs by theexcimer laser. The hydrogen peroxide systems including AOSEPT, Oxysept, Contopharma-peroxide-system were neutralized first, then applied three times on the epithelial wounds. The neutralizing procedures included the agents, duration, doses and containers following the instructions accompanied in the package of the solutions. The multipurpose care solutions includihng Opti-free, Hexidin, Bausch & Lomb Sensitive Eyes were applied directly as well. The healing scores were ranked 24 hours later with the fluorescin stain. There were no statistical differences between the healing scores of each experiment group and control group performed with Mann-Whitney rank-sum test. None of the lens care solutionjs retarded the corneal epithelial wound healing when compared with the BSS. The contact lenses treated with above solutions may be safely applied on eye without rinsing.

並列關鍵字

hydrogel contact lens ； corneal epithelium ； lens care soultion