- 期刊
Effects of Toona sinensis Leaf Extract on Lipolysis in Differentiated 3T3-L1 Adipocytes
探討香椿葉萃取物對3T3-L1脂肪細胞脂肪分解的作用
摘要
本實驗主要是探討香椿葉以50%酒精抽取的萃取物對3T3-L1脂肪細胞之脂肪分解作用的效果。脂肪分解作用的能力主要是測量細胞釋放甘油到培養液的含量而定。結果發現香椿葉萃取物會隨著處理時間及濃度的增加而促進甘油之釋放量。細胞經過不同濃度香椿葉萃取物（0.001、0.01及0.1 mg/mL）處理6小時後，細胞甘油釋放量由控制組的99 nmol/mg protein 明顯增加到127、144及154 nmol/mg protein，香椿葉萃取物促進脂肪分解的作用會被dibutyryl cAMP 及蛋白質酵素C抑制劑（calphostin C）所抑制。但是香椿葉萃取物促進脂肪分解作用不會被蛋白質合成抑制劑（cycloheximide）、cytochrome P-450 抑制劑（econazole）、lipoxidase抑制劑（baicalein）及cyclooxygenase抑制劑（indomethacin）所抑制。由以上結果顯示由50%酒精溶液所萃取的香椿葉促進3T3-L1脂肪細胞的脂肪分解作用會被cAMP所抑制並與蛋白質酵素C的途徑有關。
並列摘要
The effect of substances extracted from Toona sinensis leaves with 50% alcohol solution on lipolysis was investigated in cultured 3T3-L1 differentiated adipocytes. The amount of glycerol released from cells into culture medium was used to measure lipolysis activity. Glycerol release was increased by Toona sinensis leaf extract in a dose-dependent and time-dependent manner. Following treatment of 3T3-L1 adipocyte cells with various concentration of Toona sinensis leaf extract (0.001, 0.01, and 0.1 mg/mL) for 6 hours, the amounts of glycerol released from 3T3-L1 cells increased from a control value of 99 nmol/mg protein to 127,144, and 154 nmol/mg protein, respectively. The lipolytic effect of Toona sinensis leaf extract was not inhibited by pretreatment of cells with cycloheximide, econazole, baicalein, or indomethacin. However, the lipolytic activity induced by Toona sinensis leaf extract was diminished by dibutyryl cyclic adenosine-5’-monophosphate (dibutyryl cAMP) and the protein kinase C inhibitor calphostin C. These results indicate that the lipolytic effect induced by Toona sinensis leaf substances may be involved in the protein kinase C pathway and may be down-regulated by cAMP.
延伸閱讀
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