- 期刊
Antimicrobial Effect of Extracts of Cruciferous Vegetables
十字花科植物萃取物之抗菌效用
摘要
The cruciferous vegetables cauliflower, broccoli, cabbage, Chinese radish, Chinese kale, and Chinese kitam were used in this study to prepare water-soluble and methanol-water extracts. Crude protein extracts were also obtained by diethylaminoethyl (DEAF) anion exchange chromatography. Water-soluble polysaccharides were prepared by ethanol precipitation followed by ultrafiltration. The antimicrobial effects of all these extracts were evaluated against Gram-positive bacteria, Gram-negative bacteria, and yeast. Crude protein extracts exhibited the greatest antimicrobial activity in monoculture experiments. The antimicrobial effects of cruciferous vegetables were also studied by steeping beef, carrot, and celery in chlorine (10ppm) or citric acid solution (1%) containing the crude protein extract (500ppm) for different time periods. Total aerobic plate counts and coliform counts on these foods decreased significantly after 10 minutes in all steeping solutions (p＜0.05).
並列摘要
The cruciferous vegetables cauliflower, broccoli, cabbage, Chinese radish, Chinese kale, and Chinese kitam were used in this study to prepare water-soluble and methanol-water extracts. Crude protein extracts were also obtained by diethylaminoethyl (DEAF) anion exchange chromatography. Water-soluble polysaccharides were prepared by ethanol precipitation followed by ultrafiltration. The antimicrobial effects of all these extracts were evaluated against Gram-positive bacteria, Gram-negative bacteria, and yeast. Crude protein extracts exhibited the greatest antimicrobial activity in monoculture experiments. The antimicrobial effects of cruciferous vegetables were also studied by steeping beef, carrot, and celery in chlorine (10ppm) or citric acid solution (1%) containing the crude protein extract (500ppm) for different time periods. Total aerobic plate counts and coliform counts on these foods decreased significantly after 10 minutes in all steeping solutions (p＜0.05).
延伸閱讀
- 林俊義、黃秀華（1995）。十字花科蔬菜抗根瘤病之研究。中華農業研究，44(4)，420-429。https://doi.org/10.29951/JARC.199512.0005
- Suwanmanon, K., Rachtanapun, C., Surapat, S., Chiu, C. H., & Guu, Y. K. (2010). 植物萃取物抑制病原菌之探討. 臺灣農業化學與食品科學, 48(4), 197-203. https://doi.org/10.6578/TJACFS.2010.024
- 吳雅芳、陳紹崇、鄭安秀（2005）。十字花科蔬菜黑腐病菌血清偵測技術。臺南區農業改良場研究彙報，(46)，10-19。https://doi.org/10.29558/XLZY.200512.0002
- 楊德琳（2000）。十字花科蔬菜中??衍生物對於解毒酵素?還原?的調節作用〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714395215
- 謝廷芳、黃晉興、謝麗娟、胡敏夫、柯文雄（2005）。植物萃取液對植物病原真菌之抑菌效果。植物病理學會刊，14(1)，59-66。https://doi.org/10.6649/PPB.200503_14(1).0007