- 期刊
Use of Caveolin-1, Thyroid Transcription Factor-1, and Cytokeratins 7 and 20 in Discriminating between Primary and Secondary Pulmonary Adenocarcinoma from Breast or Colonic Origin
利用Caveolin-1, TTF-1, CK7和CK20來區分原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌
摘要
本篇研究的目的首先是要比較caveolin-1、TTF-1、CK7和CK20個別在原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌的表現並且調查這些因子單獨使用或是聯合這些因子的表現比較能區分原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌。此次研究所選定的49個肺腺癌樣本，分別為30個原發性肺腺癌和19個轉移性肺腺癌（包括有9個來自乳房轉移和10個來自大腸轉移之肺腺癌樣本）。我們利用免疫組識化學染色的方法來評估caveolin-1、TTE-1、CK7和CK20的表現。當染色結果呈現CK7陽性/CK20陰性並且TTF-1為陽性或caveolin-1陰性時最有可能的是原發性肺腺癌。當呈現CK7陽性/CK20陰性及TTF-1陰性或caveolin-1陽性最可能是來自乳房轉移的肺腺癌。若染色結果為CK20陽性/CK7陰性和TTF-1陰性或caveolin-1陽性則最可能是來自大腸轉移的肺腺癌。本篇研究結果顯示，單獨使用caveolin-1、TTF-1、或CK7/CK20並不能有效的區別原發性肺腺癌和來自乳房或大腸的轉移性肺腺癌。聯合這些因子時敏感性較高比較能區分肺腺癌是原發性的或是從乳房、大腸轉移來的。
並列摘要
The objectives of this study were firstly to compare the immunostaining patterns of antibodies against caveolin-1, thyroid transcription factor-1(TTF-1), cytokeratin7(CK7)and cytokeratin 20 (CK20) in primary and secondary pulmonary adenocarcinomas of breast or colonic origin, and secondly, to investigate their use alone and in combination, in distinguishing between primary and secondary lung adenocarcinomas from breast or colonic origin. Of the 49 lung Adenocarcinoma specimens that were enrolled in this study, 30 were primary pulmonary adenocarcinomas, and 19 (9, breast origin; 10, colonic origin) were metastatic pulmonary carcinomas. Immunohistochemical staining was used to detect the expression of caveolin-1, TTF-1, CK7, and CK20. Primary pulmonary adenocarcinoma most often had the CK7-positive/CK20-negative immunohistochemical phenotype and was either TTF-1 positive or caveolin-1 negative. Secondary pulmonary adenocarcinoma of breast origin most often had the CK7-positive/CK20-negative immunohistochemical phenotype and was either TTF-1 negative or caveolin-1 positive, while secondary pulmonary adenocarcinoma of colonic origin most often had the CK20-positive/CK7-negative immunohistochemical phenotype and was either TTF-1 negative or caveolin-1 positive. The results suggest that caveolin-1, TTF-1, or CK7/CK20 alone did not distinguish reliably between primary and secondary pulmonary adenocarcinomas originating from breast or colon. The use of a panel of antibodies that includes TTF-1, caveolin-1, and CK7/CK20 may have higher sensitivity in discriminating between primary adenocarcinomas and metastatic lung adenocarcinomas from breast or colonic origin.
延伸閱讀
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