This paper provides analysis methods for evaluating the time of 50% up-migrated fish. Some experiments were done for the up-migrated fishes including Varicorhinus barbatulus, Acrossocheilus paradoxus, time duration was 24 hours. The slope of tested fishway (modified boat-passage fishway/modified Larinier fishway) was 1/10, width was l.0m, length was 12.0m, discharge was 0.032cms. Transform the cumulative percent of up-migrated fish to the value of Probit, then compute the time of cumulative 50% up-migrated fish (T50). As to the experiments, the T50 of the Varicorhinus barbatulus fish was 0.92 hours and the Acrossocheilus paradoxus fish was 1.11 hours. The average speed of the up-migration of the Varicorhinus barbatulus fish was faster than the Acrossocheilus paradoxus fish.