The Effect of Diuretics on Sodium Transport Across the Mouse Intestine

數種利尿劑對小白鼠小腸運轉鈉離子之影響

張立人(L. R. Chang)

《The Chinese Journal of Physiology》 22卷1期 (1975/12) Pp. 39-46

摘要

本實驗探討三種利尿劑對小白鼠小腸運轉納離子之影響。結果顯示Ethacrynic caid可減低黏膜至漿膜之運轉，但不影響漿膜至黏膜之運轉，因而使鈉離子運轉淨値減小。Ethacrynic acid或Amiloride對小腸運轉納離子之作用較不明顯，唯在浴液中加有葡萄糖時，該二種利尿劑對小腸運轉鈉離子方具抑制作用。因此推論小白鼠小腸之運轉鈉離子可能有二種不同機轉，(一)鈉離子之運轉需要葡萄糖或其他作用物之存在，此運轉過程可被Furosemide和Amiloride抑制，(二)鈉離子之運轉無需葡萄糖或其他作用物之存在，此運轉過程可被Ethacrynic acid抑制。

並列摘要

Chang, L. R. The effect of diuretics on sodium transport across the mouse intestine. Chinese J. Physiol., 22(1): 39-46, 1975. The effects of three natriuretic agents on Na transport across the mouse intestine were determined. It was found that ethacrynic acid at a concentration of 1 mM, markedly reduced the net transmural transport of Na ion by decreasing the mucosa-to-serosa flux with little or no effect on serosa-to-mucosa flux. Furosemide or amiloride at a concentration of 1 mM produced similar but less profound effects. A reduction of net 22^Na flux was observed in the presence of either diuretic, but with furosemide and amiloride this inhibitory effect observed only if glucose was present in the bathing solution. It is, therefore, concluded that the intestinal transport of Na ion involves two processes, one is glucose or substrate-dependent, and the other glucose or substrate-independent. Ethacrynic acid inhibits the substrate-independent process while furosemide and amiloride affect only the substrate-dependent process of Na transport.

並列關鍵字

Sodium fluxes ； Potential difference ； Short-circuit-current ； Mouse intestine ； Ethacrynic acid ； Furosemide ； Amiloride