- 期刊
A Primary Cell-Culture System for Physiological Studies of Adrenocortical Function
並列摘要
We used guinea-pig adrenal tissue to develop a primary culture, enriched with zona fasciculate (ZF) cells. In a continuous culture up to 2 weeks, the cells maintained the characteristic of glucocortical function by producing cortisol as the final steroidogenic product and secreting it into culture medium. When culture medium, which was replaced at 24-hr intervals, was assayed for cortisol, the basal steroidogenic function peaked on day 5 and then declined. In response to 24-hr treatment with the bioactive adrenocorticotropic hormones (ACTH) on day4, production of cortisol was stimulated and prolonged, and also cells were morphologically hypertrophic. This in vitro system provides a convenient laboratory method which can be used for studying adrenocortical functionunder a possibly physiological condition.
延伸閱讀
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