- 期刊
Inhibitory Effect of Evodiamine on Aldosterone Release by Zona Glomerulosa Cells in Male Rats
並列摘要
Evodiamine is a bioactive alkaloid extracted from a Chinese herb named Wu-Chu-Yu, which possesses thermoregulatory, analgesic, and cardiovascular effects. Some studies have demonstrated that evodiamine reduces blood pressure through acting on endothelium and smooth muscle cells to produce a vasodilatory effect, but whether it affects aldosterone secretion is unclear. The purpose of this study was to examine the effect of evodiamine on aldosterone release in adrenal zona glomerulosa (ZG) cells. ZG cells were isolated from the adrenal glands of adult male rats and incubated with angiotensin II (Ang II, 1x10^(-7)M) and (superscript 3)H-pregnenolone in the presence or absence of evodiamine 1×10^(-6)~l×l0^(-3) M) at 37°C for one hour. The concentration of aldosterone in the media was measured by a radioimmunoassay. The level of radioactivity incorporated into aldosterone and its precursors after incubation of ZG cells with 3H-pregnenolone was analyzed by thin-layer chromatography. The results demonstrated that evodiamine decreased the basal level of and Ang H-induced release level of aldosterone in rat ZG cells. Administration of evodiamine also decreased the level of radioactivity incorporated into 3H-corticosterone and (superscript 3)H-aldosterone following incubation of ZG cells with 3H-pregnenolone. This suggest that evodiamine affects aldosterone release in rat adrenal glomerulosa cells by acting on Ang TI-associated pathway and reducing the activity of β-hydroxylase (an enzyme which coverts deoxycorticosterone to corticosterone) during the steroidogenesis of aldosterone.
延伸閱讀
- Chen, S. M., Wang, C. C., Lin, F., & Young, T. K. (2002). Absent Effect of Zinc Deficiency on the Oxidative Stress of Erythrocytes in Chronic Uremic Rats. The Chinese Journal of Physiology, 45(1), 9-17. https://www.airitilibrary.com/Article/Detail?DocID=03044920-200203-45-1-9-17-a
- Sun, Y. F., Yen, C. H., & Lau, Y. T. (1997). Inhibitory Effect of Estrogen on Vascular Contraction From Male Rats of Different Ages. The Chinese Journal of Physiology, 40(2), 79-83. https://www.airitilibrary.com/Article/Detail?DocID=03044920-199706-40-2-79-83-a
- Tsai, S. C., Kau, M. M., & Wang, P. S. (2019). Effects of Amphetamine on the Release of Corticosterone and Aldosterone in Rat Adrenocortical Cells. Adaptive Medicine, 11(4), 127-132. https://doi.org/10.4247/AM.2019.ABJ239
- González, R., Romay, C., Borrego, A., Hernández, F., Merino, N., Zamora, Z., & Rojas, E. (2005). Lipid Peroxides and Antioxidant Enzymes in Cisplatin-Induced Chronic Nephrotoxicity in Rats. Mediators of Inflammation, 2005(), 139-143. https://doi.org/10.1155/MI.2005.139
- Chen, H. I., Cheng, S. Y., & Jen, C. J. (1999). Chronic Exercise Enhances Vascular Responses to Clonidine in Rats by Increasing Endothelial α2-Adrenergic Receptor Affinity. The Chinese Journal of Physiology, 42(2), 61-66. https://www.airitilibrary.com/Article/Detail?DocID=03044920-199906-42-2-61-66-a