Anti-inflammatory Effect of Hydroxyethylpuerarin on Focal Brain Ischemia/Reperfusion Injury in Rats

The objective of this study is to investigate the anti-inflammatory effect of hydroxyethylpuerarin on focal brain ischemia injury in rats and to explore its mechanisms of action. After 24 h of reperfusion following 2 h of cerebral ischemia, the infiltration of neutrophils was observed by myeloperoxidase (MPO) activity determination, the expression of intercellular adhesion molecule-1 (ICM-1) was observed by western blot and reverse transcriptase-polymerase chain reaction(RT-PCR) analysis, and the nuclear translocation and DNA binding activity of nuclear factor-кB (NF-кB)were observed by western blot and electrophoretic mobility shift assay (EMSA). The results showed that hydroxyethylpuerarin could obviously inhibit the MPO activity and ICAM-1 expression following 2 hours of ischemia with 24 hours of reperfusion. The nuclear translocation and DNA binding activity were also decreased by hydroxyethylpuerarin treatment. These results suggested that hydroxyethylpuerarin could inhibit neutrophil-mediated inflammatory response after brain ischemia reperfusion in rats. This effect may be mediated by down-regulation of ICAM-1 and NF-кB activity.

並列關鍵字

hydroxyethylpuerarin ； myeloperoxidase ； intercellular adhesion molecule-1 ； nuclear factor-x B ； brain ischemia injury

參考文獻

Albert, S.,Baldwin, J.(1996).The NF-kB and I kB proteins: new discoveries and insights.Annu. Rev. Immunol..14,649-681.

Google Scholar

Barnes, P.J.,Karin, M.(1997).Nuclear factor-kB: a pivotal transcription factor in chronic inflammatory disease.N. Engl. J. Med..336,1066-1071.

Google Scholar

Connolly, E.S.,Winfree, C.J.,Springer T.A,Naka, Y.,Liao, H.,Yan, S.D.,Stem, D.M.,Solomon, R.A.,Gutierrez-Ramos, J.-C.,Pinsky, D.J.(1996).Cerebral protection in homozygous null ICAM-1 mice after middle cerebral artery occlusion: role of neutrophil adhesion in the pathogenesis of stroke.J. Clin. Invest..97,209-216.

Google Scholar

Huang, H.-D.,Liu, Z.-H.,Zhu, X.-J.,Chen, Z.H.,Li, L.S.(2002).Inhibition of mycophenolic acid on NF-kB activity in human endothelial cells.Acta Pharmacol. Sin..23,649-653.

Google Scholar

Longa, E.L.,Weinstein, P.R.,Carlson, S.,Cummins R.(1989).Revesible middle cerebral artery occlusion without craniectomy in the rats.Stroke.20,84-91.

Google Scholar

延伸閱讀

Wang, Z. Y., Wei, X. B., Chen, L., Liu, P., Wang, L. X., Zhang, B., Sun, X., & Zhang, X. M. (2007). Neuroprotective Effects of Hydroxyethylpuerarin against Focal Cerebral Ischemia-Reperfusion in Rats. The Chinese Journal of Physiology, 50(5), 211-216. https://www.airitilibrary.com/Article/Detail?DocID=03044920-200710-50-5-211-216-a
Jiang, Y., Wei, N., Zhu, J., Lu, T., Chen, Z., Xu, G., & Liu, X. (2010). Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat. Mediators of Inflammation, 2010(), 391-400. https://doi.org/10.1155/2010/372423
Chang, H. C., Yang, Y. R., Wang, P. S., Kuo, C. H., & Wang, R. Y. (2010). Effects of Insulin-Like Growth Factor 1 on Muscle Atrophy and Motor Function in Rats with Brain Ischemia. The Chinese Journal of Physiology, 53(5), 337-348. https://doi.org/10.4077/CJP.2010.AMK080
吳姿瑩（2012）。Effect of Lipopolysaccharide-induced Systemic Inflammation on Hyperoxia-exposed Newborn Rats〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://doi.org/10.6831/TMU.2012.00125
Pin-Jie Huang、Xiao-Liang Gan、Jian-Pei Liu、De-Zhao Liu、Yan-Ling Wang、Zi-Qing Hei（2014）。Effects of Anti-Histamine Treatment on Liver Injury Triggered by Small Intestinal Ischemia Reperfusion in Rats。The Chinese Journal of Physiology，57(5)，271-278。https://doi.org/10.4077/CJP.2014.BAC230

國際替代計量

Anti-inflammatory Effect of Hydroxyethylpuerarin on Focal Brain Ischemia/Reperfusion Injury in Rats

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