Hepatoprotective Effects of Traditional Chinese Medicine on Liver Fibrosis from Ethanol Administration following Partial Hepatectomy

Jia-Ping Wu ； Tsung-Jung Ho ； Chin-Chuan Tsai ； Yu-Lan Yeh ； Chien-Chung Lin ； Kuan-Ho Lin ； Dennis Jine-Yuan Hsieh ； Li-Mien Chen ； Lung-Fa Pan ； Chih-Yang Huang

《The Chinese Journal of Physiology》 預刊文章 Pp. 1-11

https://doi.org/10.4077/CJP.2015.BAD339

並列摘要

The aim of this study was to establish the effective hepatoprotective properties of traditional Chinese medicines in fibrotic rat liver regeneration after partial hepatectomy (PHx). Fibrosis was induced in rats by ethanol (EtOH, 5 ml/kg) administration for 6, 24, 72, and 168 h. The rats were then fed four traditional Chinese medicines (1g/kg/day, Codonopsis pilosula (CP), Salvia miltorrhiza Bunge (SMB), Bupleurum kasi (BK), and Elephantopus scaber L (ESL) to Spraque-Dawley rats for 6, 24, 72 and 168 h, respectively. Surgical 70% cirrhotic fibrosis PHx was then conducted at 6, 24, 72 and 168 h. The effects on liver regeneration were examined to estimate and measure HGF, FAK, Cyclin D1, Cyclin E, and pRb protein expression using western blotting analysis. Cyclin D1, MMP-2, MMP-9, TIMP-1, TIMP-2 and TIMP-3 mRNA by RT-PCR were analyzed in cirrhotic fibrosis rats. TGF-β1, Cyclin D1, Cyclin E, pRb and E2F mRNA expression levels were determined in fibrotic rats following PHx using RT-PCR. We found elevated GOT, GPT, ALP, γ-GT, GSH, NPSH and total- bilirubin in serum after 6 h ethanol administration. These levels were progressively decreased over 168 h. Total protein and albumin were reduced in serum after 6 h administration and then progressively increased. In contrast, tissues disorder histology and morphology were determined in liver sections. After rats were fed traditional Chinese medicines we found that SMB extraction not only induced HGF, FAK, Cyclin D1, and pRb protein expression and Cyclin D1 mRNA increases, but also reduced MMP-2 and MMP-9 after 24 and 72 h post injury. In the cell cycle S phase the Cyclin E protein expression was increased by ESL. CP induced TIMP-1, TIMP-2 and TIMP-3 mRNA increases in fibrotic rats. We detected liver regeneration in fibrotic rats. We also found that the liver regeneration index increased from 6 to 168 h post PH. After 168 h fibrotic liver regeneration rats exhibited reduced TGF-β1 mRNA expression and enhanced Cyclin D1, Cyclin E, pRb and E2F mRNA expression. TCMs play a crucial role in the early mediating process in fibrotic rat liver regeneration after PHx.

並列關鍵字

fibrotic ； hepatocyte growth factor ； liver regeneration ； partial hepatectomy ； traditional Chinese medicine

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