Effect of BayK 8644 on [Ca^(2+)](subscript i) and Viability in PC3 Human Prostate Cancer Cells

Zhen-Rung Lai ； Chiang-Ting Chou ； Shiuh-Inn Liu ； Wei-Zhe Liang ； Jong-Khing Huang ； Chung-Ren Jan

《The Chinese Journal of Physiology》 預刊文章 Pp. 11-18

並列摘要

The effect of BayK 8644 on cytosolic Ca^(2+) concentrations ([Ca^(2+)](subscript i)) and viability in PC3 human prostate cancer cells was explored. Fura-2 was applied to measure [Ca^(2+)](subscript i). BayK 8644 at 1-50 μM induced a [Ca^(2+)](subscript i) rise concentration-dependently. The response was reduced by removing extracellular Ca^(2+). BayK 8644-evoked Ca^(2+) entry was inhibited by nifedipine, econazole, SK&F96365, and protein kinase C modulators. In Ca^(2+)-free medium, incubation with the endoplasmic reticulum Ca^(2+) pump inhibitor 2, 5-di-tert-butylhydroquinone (BHQ) abolished BayK 8644-induced [Ca^(2+)](subscript i) rise. Inhibition of phospholipase C did not alter BayK 8644-induced [Ca^(2+)](subscript i) rise. BayK 8644 killed cells in a concentration-dependent manner, which was not reversed by chelating cytosolic Ca^(2+) with 1, 2-bis (2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid/acetoxy methyl (BAPTA/AM). Collectively, in PC3 human prostate cancer cells, BayK 8644 induced a [Ca^(2+)](subscript i) rise by evoking phospholipase C-independent Ca^(2+) release from the endoplasmic reticulum and Ca^(2+) entry via protein kinase C-sensitive store-operated Ca^(2+) channels (and/or T-type Ca^(2+) channels). At high concentrations, BayK 8644 caused cell death.

並列關鍵字

BayK 8644 ； Ca^(2+) ； PC3

參考文獻

Bootman, M.D.,Berridge, M.J.,Roderick, H.L.(2002).Calcium signalling: more messengers, more channels, more complexity.Curr. Biol..12,R563-R565.

Chang, H.C.,Cheng, H.H.,Huang, C.J.,Chen, W.C.,Chen, I.S.,Liu, S.I.,Hsu, S.S.,Chang, H.T.,Wang, J.K.,Lu, Y.C.,Chou, C.T.,Jan, C.R.(2006).Safrole-induced Ca2+ mobilization and cytotoxicity in human PC3 prostate cancer cells.J. Recept. Signal Transduct. Res..26,199-212.

Chang, H.C.,Huang, C.C.,Huang, C.J.,Cheng, J.S.,Liu, S.I.,Tsai, J.Y.,Chang, H.T.,Huang, J.K.,Chou, C.T.,Jan, C.R.(2008).Desipramine-induced apoptosis in human PC3 prostate cancer cells: activation of JNK kinase and caspase-3 pathways and a protective role of [Ca2+]i elevation.Toxicology.250,9-14.

Cheng, J.S.,Shu, S.S.,Kuo, C.C.,Chou, C.T.,Tsai, W.L.,Fang, Y.C.,Kuo, L.N.,Yeh, J.H.,Chen, W.C.,Chien, J.M.,Lu, T.,Pan, C.C.,Cheng, H.H.,Chai, K.L.,Jan, C.R.(2011).Effect of diindolyl-methane on Ca2+ movement and viability in HA59T human hepatoma cells.Arch. Toxicol..85,1257-1266.

Curl, C.L.,Delbridge, L.M.,Wendt, I.R.(2008).Sex differences in cardiac muscle responsiveness to Ca2+ and L-type Ca2+ channel modulation.Eur. J. Pharmacol..586,288-292.