- 期刊
Effect of Carbon Source on Inclusion Body Formation upon Overproduction of Periplasmic Penicillin Acylase in Escherichia coli
碳源對於大腸桿菌中大量生產Penicillin Acylase之影響
摘要
本文主要探討不同糖類添加對Penicillin Acylase之生產與包涵體形成之效應。
關鍵字
無資料並列摘要
The effect of carbon supplementation on penicillin acylase (PAC) production and inclusion body formation was investigated. The host effect was also examined by comparing culture performances using HB101 or its derived mutant, MD△P7, as the expression host. Among several carbons tested, glycerol was found to be optimum for PAC production. PAC activity was increased and the amount of inclusion bodies was reduced upon glycerol supplementation. The effete of carbon source on inclusion body formation appears to be strain-dependent. With the optimization on the culture parameters (i. e., host and carbon effects), volumetric PAC activity could be significantly improved at more than fourfold that of the control experiment. This information is useful for developing expression strategies for optimization of PAC production.
延伸閱讀
- 劉玉雯（2015）。碳源對畢赤酵母菌表現重組蛋白hG-CSF之影響〔碩士論文，國立宜蘭大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0046-0210201517210500
- 黃盈蓁（2012）。不同碳系電極對大腸桿菌微生物燃料電池性能影響之研究〔碩士論文，國立清華大學〕。華藝線上圖書館。https://doi.org/10.6843/NTHU.2012.00170
- 黃雅惠（2006）。碳布壓縮量對仿生物結構流道之燃料電池的影響〔碩士論文，元智大學〕。華藝線上圖書館。https://doi.org/10.6838/YZU.2006.00094
- 李珮芸（2011）。外加碳源對生物薄膜反應器處理低碳氮比高科技業廢水之影響〔碩士論文，國立交通大學〕。華藝線上圖書館。https://doi.org/10.6842/NCTU.2011.01086
- Wu, C. Y., Liang, Z. C., Lu, C. P., & Wu, S. H. (2008). 碳氮源對浸漬培養金頂側耳胞外多醣之生成及碳水化合物組成之影響. 藥物食品分析, 16(2), 61-67. https://www.airitilibrary.com/Article/Detail?DocID=10219498-200804-16-2-61-67-a