The purpose of the study is to understand the effect of compresed GDL(Gas diffusion layer) on the performance of Bio-cell Fuel cell. In our experiments, we tested efficiency of fuel cell on several compression ratios of GDL, which are 71.4%, 42.9% and 14.3%, with gasket thickness of 0.1mm, 0.2mm and 0.3mm to get optimal efficiency for fuel cell. Teflon gaskets are used to control compression ratios of gas diffusion layers. The compression ratios are direct proportional to thickness of gasket, and inverse proportional to porosity. Thicker gasket also leads to higher pressure difference and higher electrical conductivity. The test of fuel cell is under various temperatures with pressure gauge to obtain parameters at airway of fuel cell. The purpose is to analyze the variation under different test parameters. The results of the study indicates that optimal efficiency of fuel cell will occurred at compression ratio when carbon layer is 0.2mm.