Hydrolysis of Soybean Oil by Surfactant - Coated Lipase in Organic Solvents

界面活性劑覆蓋脂解酵素進行脂解反應有諸多優點，大豆油水解後所得脂肪酸甚具營養價值，並有抗心血管疾病之效果。本研究使用Candida rugosa來源之脂解酵素製備界面活性劑包覆脂解酵素（SCL）以催化大豆油之水解反應。探討製備最佳SCL之條件，如界面活性劑的種類，界面活性劑與脂解酵素之重量比，緩衝溶液之pH值等，此等因素影響SCL之收率，蛋白質含量，包覆率及水解能力甚鉅，實驗結果以HLB及log P（疏水性）來解釋。製備SCL之最佳條件為pH５，界面活性劑／脂解酵素重量比４／１。在此條件下製備之SCL其催化活性為粉末酵素之３．６倍，獲致最高水解率之反應條件為３７°C，緩衝溶液之pH值為７．０，使用有機溶劑異辛烷。以此條件水解率在１２．３小時可達９６．２８％。

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並列摘要

The advantages of coating surfactants onto the lipases for catalyzing lipolytic reactions in organic solvents motivated this investigation. The fatly acids obtained from hydrolysis of soybean oil are of nutritional value and are beneficial to the cardiovascular system. A lipase from Candida rugosa was used to prepare a surfactant-coated lipase (SCL) for catalyzing the hydrolysis of soybean oil. The conditions for obtaining a superior SCL, including the kind of surfactant, weight ratio of surfactant / lipase and pH of the buffer solution were investigated. These factors significantly affected the catalytic power, yield of SCL, protein content and the coverage of lipase by surfactant. The results obtained were explained by the hydrophile-lipophile balance (HLB) of the surfactant and the log P (hydrophobicity) of the organic solvent. The optimum conditions for preparing SCL for enhancing the hydrolysis of soybean oil were pH=5.0 and weight ratio of Span 60 and lipase= 4/1. The catalytic activity of the SCL was 3.6 fold greater than powdered lipase. The optimum conditions for hydrolysis were investigated by varying the organic solvent as well as the temperature and pH of the milieu. SCL exhibited the highest hydrolysis rate at 37°C in a buffer solution of pH7.0 with isooctane. A conversion rate of 96.28% was reached in 12.3 hr.

並列關鍵字

Surfactant-coated Lipase ； Soybean Oil ； Hydrolysis Conversion ； Organic Solvent

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Hydrolysis of Soybean Oil by Surfactant - Coated Lipase in Organic Solvents

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