以氫氧化鈉或脂解酶催化甘油解法製備富含雙酸甘油酯大豆油之物理化學特性之探討

Study on the Physical and Chemical Properties of Diacylglycerol-Enriched Soybean Oil Prepared by Sodium Hydroxide- or Lipase- Catalyzed Glycerolysis

蔡羽豐(Yu-Feng Tsai) ； 徐錫樑(Shyi-Liang Shyu)

《臺灣農業化學與食品科學》 58卷6期 (2020/12) Pp. 139-146

https://doi.org/10.6578/TJACFS.202012_58(6).0001

摘要

本研究主要探討大豆油以氫氧化鈉催化甘油解法（SHCG）與脂解酶催化甘油解法（LCG）製備雙酸甘油酯（diacylglycerol, DAG）。將大豆油製備之粗DAG以管柱區分進行純化，得到富含雙酸甘油酯大豆油（Diacylglycerol-enriched soybean oil, DESO），並測定其理化性質。結果顯示，SHCG或LCG製備之DESO，其熔點、黏度、折射率及介電常數皆比大豆油高。然而，其發煙點比大豆油略低。在酸價、過氧化價及硫代巴比妥酸價，大豆油與DESO並無明顯差異，因此，SHCG或LCG皆可製得品質良好的DESO。DESO以X光繞射分析其晶型，SHCG製備之DESO，其晶型主要為β型態（4.6 Å）組成，而LCG製備之DESO，其晶型為β型態（4.6 Å）與β'型態（4.2 Å）所組成。因此，SHCG製備之DESO比LCG製備之DESO穩定。

關鍵字

雙酸甘油酯 ； 大豆油 ； 氫氧化鈉催化甘油解法 ； 脂解酶催化甘油解法

並列摘要

This study was to investigate the diacylglycerol (DAG) prepared from soybean oil with sodium hydroxide catalyzed glycerolysis (SHCG) and lipase catalyzed glycerolysis (LCG). The crude DAG prepared from soybean oil which was purified by the column fractionation to obtain diacylglycerol-enriched soybean oil (DESO) and determined its physicochemical properties. The results showed that melting point, viscosity, refractive index and dielectric constant of DESO prepared by SHCG or LCG were all higher than that of soybean oil while the smoke point was slightly lower. There was no significant difference between soybean oil and DESO in acid value, peroxide value and thiobarbituric acid value. Therefore, DESO prepared by SHCG or LCG had a good quality. The crystal polymorphism of DESO was determined by X-ray diffractometer. The main crystal form of DESO prepared by SHCG was β-form (4.6 Å) while the DESO prepared by LCG was β-form (4.6 Å) and β'-form (4.2 Å). Therefore, DESO prepared by SHCG was more stable than DESO prepared by LCG.

並列關鍵字

Diacylglycerol ； Soybean oil ； Sodium hydroxide catalyzed glycerolysis ； Lipase catalyzed glycerolysis