本研究探討在1公升加壓二氧化碳反應器中,以不同操作條件下,異澱粉?水解支鏈澱粉之反應情形。實驗結果顯示在加壓二氧化碳之下,攪拌破壞酵素活性情形顯著,而且由於高壓二氧化碳的泬解度,改變了系統的酸鹼值,進而降低反應速率。故適當加以調整攪拌速率與系統酸鹼值,異澱粉?在超界二氧化碳中之水解支鏈澱粉反應與常壓系統比較,在濃度較高時具有較佳的反應速率。
Hydrolysis of amylopectin by a debranching enzyme, isoamylase, was investigated under pressurized carbon dioxide in a reactor of one liter for various agitation rates and pH values. The experimental results indicated that agitation significantly inactivated the enzyme with pressurized carbon dioxide. The pH shift due to higher solubility of carbon dioxide in the system also decreased the rate. With proper tuning of agitation rate and pH, the enzyme hydrolysis reaction under supercritical carbon dioxide demonstrated better result than that under atmospheric conditions at higher substrate concentration.