Heparin Modulates Human Osteosarcoma (MG-63) Activities on Collagen-Coated Substrata

膠原蛋白是細胞外基質的主要蛋白質，也因為其優越的生物相容性，成為受歡迎的生醫材料。膠原蛋白被廣泛於外科手術縫線、繃帶、藥物傳輸用水膠、傷口敷材，也應用於新近興起的組織工程領域。如果對於細胞和膠原蛋白基材，以及其他的細胞外基質成分的交互作用有深一層的瞭解，應該對於設計組織工程用鷹架有進一步的幫助。在本研究中，我們探討肝素在膠原蛋白基材上，對於骨母細胞的影響。膠原蛋白(0.1mg/mL)和不同濃度的肝素（0.02mg/mL、0.1mg/mL和0.5mg/mL）混合後，塗佈在細胞培養盤中。我們發現低濃度的肝素(0.02mg/mL)能夠提高骨母細胞的附著和伸展，但是對於細胞生長和鹼性磷酸的活性沒有影響。相反地，高濃度的肝素（0.1和0.5mg/mL）降低骨母細胞的附著和伸展，同時也減少細胞的繁殖。本研究的結果顯示少量的肝素可以增加細胞的貼附與伸展，但是高濃度的肝素並不適合細胞培養。

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並列摘要

Collagen, the major extracellular matrix component, is a popular biomaterial due to its excellent biocompatibility. Collagen is widely used in sutures, corneal bandages, drug delivery gels, and as wound dressings, as well as in a newly emerging interdisciplinary biomedical field, tissue engineering. A better understanding of the interaction between cells and collagen substrata combining other ECM components should allow for a more rational scaffold design for tissue engineering. In this study, we explored the effects of heparin on the behavior of osteoblast-like cell lines (MG-63) on collagen-based substrates. Collagen mixture (0.1mg/mL) with heparin at different concentrations (0.02mg/mL, 0.1mg/mL, and 0.5mg/mL) was coated onto tissue culture polystyrene plates. We found that low concentration of heparin (0.02mg/mL) enhanced cell adhesion and spreading on collagen-coated plates, but did not influence cell growth and alkaline phosphatase activity. On the other hand, high doses of heparin (0.1 and 0.5mg/mL) decreased cell adhesion and spreading, as well as cell proliferation. Our results suggest that a small amount of heparin might support osteoblast attachment and spreading although high heparin concentration is not suitable for cell culture.

並列關鍵字

Collagen ； Heparin ； Osteoblast ； Cell adhesion

國際替代計量

Heparin Modulates Human Osteosarcoma (MG-63) Activities on Collagen-Coated Substrata

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