Investigating Protein Crossflow Ultrafiltration Mechanisms Using Interfacial Phenomena

蛋白質掃流超過濾機制之界面現象解析

童國倫(Kuo-Lun Tung) ； 胡哲嘉(Che-Chia Hu) ； 李佳玲(Chia-Ling Li) ； 荘清榮(Ching-Jung Chuang)

《Journal of the Chinese Institute of Chemical Engineers》 38卷3&4期 (2007/07) Pp. 303-311

摘要

本研究之目的在於藉由蛋白質－蛋白質與蛋白質－薄膜間的界面現象作用解析，探討蛋白質在薄膜超過濾操作中之分離機制。本研究以實驗為主，採用聚醚碸薄膜針對牛血清蛋白、卵白蛋白、溶菌脢等三種蛋白質進行單蛋白質溶液的超過濾，分析溶液環境及薄膜特性對蛋白質超過濾程序之影響。實驗研究藉由改變溶液環境之酸鹼值、離子強度以及薄膜特性之膜孔大小、親疏水性等參數，探討蛋白質－蛋白質與蛋白質－薄膜間的界面作用，來解析蛋白質的超過濾機制及其對操作程序之影響。由實驗結果得知，蛋白質－蛋白質與蛋白質－薄膜間的靜電作用力對於蛋白質在膜面上沈積與其穿透率之影響甚鉅。改質後的疏水膜會導致蛋白質在膜面上産生嚴重吸附，進而造成蛋白質不易穿透過薄膜並減低穿透率，而此效應在溶液酸鹼值趨近蛋白質等電點時尤為顯著。

並列摘要

Protein separation mechanisms by a crossflow ultrafiltration membrane process are investingated using interfaceial phenolmena analysis of protein-protein and protein-membrane interactions. Varying operating conditions are used and membrane morphological parameters like hydrophilicity and hydrophobicity obtained from contact angle measurement. A single protein solution of bovine serum albumin (BSA) or ovalbumin (OV) or lysozyme (LY) is used in each filtration study case under various operating conditions, such as pH, ionic strength, and different polyethersulfone (PES) membranes with varying surface hydrophobicity and hydrophilicity. Under these conditions analyse of protein-membrane interfacial phenomena and protein separation filtration mechanisms are undertaken. Results indicate that electrostatic forces play an important role in protein deposition on the membrane surface and the rate of protein transmission across the membrane. Findings show that, at the proteins isoelectric point (pl), a hydrophobic membrane causes severe protein adsorption to the membrane surface, allowing a very small percentage of protein to be transported to the permeate side.

並列關鍵字

Crossflow ultrafiltration ； Protein separation ； Interfacial phenomena ； Plasma surface moditication