Isolation and Characterization of Bdellovibrio Parasitic to Gram-negative Fish Pathogenic Bacterium Aeromonas Hydrophila

寄生於格蘭氏陰性魚病細菌Aeromonas hydrophila的噬菌弧菌之篩選及其特性

蔡珊珊(San-San Tsay) ； 王西華(H. H. Wang)

《TAIWANIA》 35卷2期 (1990/06) Pp. 112-126

https://doi.org/10.6165/tai.1990.35.112

摘要

由臺北近郊分離出一株能寄生於Aeromonas hydrophila的噬菌弧菌，經多次重覆單離，並做電子顯微鏡觀察鑑定後將其定名為Bdellovibrio A-1，並探討關於影響賓主關係的因子。噬菌弧菌產生的最適溫度為25~29℃，最適酸鹼值為pH 6.5，噬菌弧菌的生長需要添加二價陽離子Ca^2+和Mg^2+。噬菌弧菌對三種魚病細菌均會造成感染，而宿主細菌的添加濃度以4x10^8 cells較適合於噬菌弧菌的生長。

並列摘要

One strain of bacteria parasite-Bdellovibrio was isolated from cuItured fish ponds in Taiwan. This strain is able to parasitize on Aeromonas hydrophila, designated as Bdellovibrio A-1. The morphology of host and parasite were examined by means of electron microscopy. The effect of medium composition, initial pH of medium, incubation temperature, addition of divalent cation and kinds and concentration of host bacteria on the growth of Bdellovibrio were investigated. The optimum temperature for the growth of Bdellovibrio in liquid medium was 25-29℃. The optimum initial pH of medium was pH 6.5. Growth of the parasite was dependent upon the addition of Ca^2+ and Mg^2+. The highest lytic action of Bdellovibrio was obtained by using a higher concentration or fish pathogenic bacteria(4x 10^8 cells/ml) as host.