Effect of capsule maturity and desiccation time on viability of Taiwan native orchid, Bletilla formosana seeds (Orchidaceae) after cryopreservation

Rung-Yi WU ； Keng-Chang CHUANG ； Ting-Fang HSIEH ； Yu-Sen CHANG

《TAIWANIA》 63卷4期 (2018/12) Pp. 345-350

https://doi.org/10.6165/tai.2018.63.345

摘要

Desiccation method has been successfully applied to the cryopreservation of Bletilla formosana seeds (Orchidaceae). This study investigated the effects of capsule maturity and desiccation time on the viability of B. formosana seeds after cryopreservation. Seeds with various degrees of maturity (60, 70, 80, 90, 100, and 110 days after pollination; DAP) were used as the test samples and dried for 0 and 24 h prior to cryopreservation. The results demonstrated that the germination percentage of the 110 DAP seeds with 24 h desiccation pretreatment was the highest (96.7%). The 110 DAP seeds were then used to further test the effects of various desiccation time (0, 2, 4, 6, 8, and 24 h) on the viability of the seeds after cryopreservation. The results demonstrated that when seeds were dried for 4-24 h prior to preservation, the water content was reduced to 10.8-0.6% (w/w), and the germination percentage (86.7-95.5%) were significant difference higher than un-desiccation treatment after cryopreservation and thawing to room temperature. The seeds of B. formosana were highly tolerant to desiccation, thus, they should be orthodox seeds and are suitable for long-term storage through cryopreservation.

關鍵字

Bletilla formosana ； Days after pollination ； Long-term preservation ； Orchid ； Orthodox seed ； Preservation

參考文獻

Chang, C., P.-G. Sung, C.-H. Chang, Y.-C. Chen and Y.-H. Lin. 2006. Seed development and storage of Bletilla formosana (Hayata) Schltr. Seed Nurs. (in Chinese with English abstract) 8:29-38.

Batty, A.L., K.W. Dixon, M. Brundrett and K. Sivasithamparam. 2001. Long-term storage of mycorrhizal fungi and seed as a tool for the conservation of endangered Western Australian terrestrial orchids. Aust. J. Bot. 49(5):619-628.

Dickie, J.B., K. May, S.V.A. Morris and S.E. Titley. 1991. The effect of desiccation on seed survival in Acer platanoides L. and Acer pseudoplatanus L. Seed Sci. Res. 1:149-162.

Engelmann, F. 2000. Importance of crop for conservation of plant genetic resources, p. 8-20. In: Engelmann, F. and H. Takagi (eds.). Cryopreservation of Tropical Plant Germplasm. JIRCAS, Tsukuba, Jpn.

Grout, B.W.W., K. Shelton and H.W. Pritchard. 1983. Orthodox behaviour of oil palm seed and cryopreservation of the excised embryo for genetic conservation. Ann. Bot. 52(3):381-384.