百香果的花葯培養

Anther Culture of Passion Fruit (Passiflora edulis)

蔡新聲(H. S. Tsay) ； 許家言(J. Y. Hsu) ； 楊翠蘋(T. P. Yang) ； 楊昭容(C. R. Yang)

《中華農業研究》 33卷2期 (1984/06) Pp. 126-131

https://doi.org/10.29951/JARC.198406.0004

摘要

本研究之目的乃利用花葯培養的技術，將百香果型質極雜之親本及雜種F1育成純系，供育種選拔之用。花葯培養之初步結果加下：
一、百香果花葯培養callus形成之最佳時期爲，小胞子發育屬四分子期至早單核期之花葯，最佳培養某爲含有6%蔗糖，2mg/l NAA及1mg/l BA之1/2 Murashige & Skoog (1962)基本鹽類固體培養基，callus之誘導與光照關係不大。
二、以多種auxins, cytokinins及ABA等值物荷爾蒙組合MS基本鹽類之數十種分化培養某，進行花葯callus分化器官之誘導，只有極少數根的形成。花葯callus之來源尚未鑑定。

並列摘要

The aim of this research is to establish the procedures of haploid plant formation from anther culture of passion fruit F1 hybrids. The results are summarized as follows:
1. Passion fruit anthers containing microspores at tetrads to early-binucleate stages were successfully cultured in 1/2 strength of Murashige and Skoog (1962) medium (except Na-Fe-EDTA) supplemented with 2mg/l NAA (Naphthaleneacetic acid), 1mg/l BA (Benzyladenine) and 6% sucrose for callus formation. Highest frequencies of callus induction were obtained from anthers with tetrads or early-uninucleate microspores. Callus could be induced under either light or dark condition.
2. The regeneration ability of anther-derived callus was tested under several kinds of auxins and cytokinins in different concentrations and combinations. None of embryoids or shoot-like structures have been derived from those calli except for some rcoty protrusions.
3. The origination of callus was unidentified.