臺灣葡萄汁液傳播性黃斑病毒病之發生

Sap-transmissible Viruses Associated with Grapevine Yellow Mottle Disease in Taiwan

楊一郎(I-Lang Yang) ； 鄧汀欽(Ting-Ching Deng) ； 陳脉紀(Moh-Jih Chen)

《中華農業研究》 35卷4期 (1986/12) Pp. 504-510

https://doi.org/10.29951/JARC.198612.0010

摘要

本省田間葡萄呈現黃斑型病徵之病毒病害，於1982年在臺中地區發現。可經汁液接種感染草本檢定植物Chenopodium amaranticolor等13種，形成局部斑點(local lesion)、黃斑(yellow spot)、斑紋(mottling)及嵌紋(mosaic)等病徵。經重複單斑分離，並由其寄主病徵反應及血清反應得到五個分離株。囘接到巨峰健全實生苗亦形成黃斑病徵。應用葡萄檢定品種(Vitis indicator)，分別得到嫁接傳播及土壤傳播之分離株，並在檢定葡萄品種上，形成黃斑或葉脈黃化病徵。各病毒之檢定植物病葉材料，經以grape fanleaf virus (GFLV), tomato ringspot virus (TmRSV), grape Bulgarian latent virus (GBLV)及grape chrome mosaic virus (GCMV)四種抗血清之ELISA測定結果，其中兩個分離株與TmRSV抗血清有正反應。經電子顯微鏡觀察結果，在病株粗汁液與組織切片中，發現直徑26-30 nm之球型病毒。由以上結果顯示此兩個分離株病毒，爲TmRSV之系統(strain)。

並列摘要

Some virus-like disorder showing yellow mottle or yellow vein or mosaic symptoms were found in some vineyards in Taichung areas in 1982. These diseases were transmitted to 13 species of herbaceous plants by sap inoculation, and showed either local lesion or yellow spot or mosaic symptoms. Five virus isolates were isolated from different single lesions according to different host reactions and symptomatology. Back inoculation to healthy grape seedlings were succeeded by mechanical transmission. Graft indexings were succeeded by use of healthy Vitis indicators, and were also confirmed as a soil-borne virus disease. Two isolates showed positive reaction to antiserum against tomato ringspot virus. Electron microscopy found spherical virus particles, 26 to 30nm in diameter in either infected leaf extract or tissue section. Accordingly, two virus isolates of these grape yellow mottle were confirmed as relative to one strain of tomato ringspot virus.