- 期刊
梨次病毒之瓜苗檢定（簡報）
Detection of Pear Viroids by Cucumber Assay (Research Note)
摘要
採集凍存本省臺中地區梨品種幼葉並製作次病毒(viroid RNAs)接種源計145個材料、經於春夏季間進行梨次病毒之瓜苗檢定(cucumber assay)結果、供試6個品種145個梨幼葉材料中、發現有27個材料在接種之胡瓜指示植物(Cucumis sativus L. cv. Sūyō呈現典型viroid病徵。在春夏季間其初期病徵顯現之潛伏期為43~58天。上述胡瓜病葉材料經重複回接在胡瓜(cv. Sūyō)結果皆得相同之病徵反應、該瓜苗指示植物在接種後6~8週呈現捲葉(leaf curling)及葉脈透化(vein clearing)等初期病徵、在後期呈現頂端黃化(top yellowing)及矮化(stunt)等特異性病徵。此項瓜苗檢定之病徵反應結果與日本佐野等報告之hop stunt viroid group相近似。
並列摘要
Pear viroid inocula were extracted from 145 leaf collections including six pear varieties cultivated in Taichung district. The pear viroids were detected in 27 out of 145 pear inoculum extractions by the viroid indexing method of cucumber assay during spring to summer season. The pear viroid induced typical viroid symptoms on cucumber indicaror plant (Cucumis sativus L.cv. Sūyō) 6-8 weeks after inoculation. The infected cucumber plants showed viroid-specific symptoms of leaf curling, vein clearing, top yellowing and stunt, which were similar to those induced by hop stunt viroid group described by Sano et al. in Japan.
延伸閱讀
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