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Differential Responses of Soybean Foliage to Simulated Acid Rain

人工模擬酸雨對大豆葉片之不同反應

摘要


本試驗目的主要是探討酸雨對大豆葉片傷害。塑膠溫室內以盆缽方式種植大豆,於開花期後(R3)開始噴施不同酸度(pH2..0,3.0,4.0和5.6)的雨水,每週噴施1公厘雨量2次,連續5週。試驗結果顯示,pH2.0酸雨噴施葉片2週後,出現白色及褐色斑點,pH3,0有少量斑點出現,至於pH4.0及5.6則不受影響。葉片電解質滲漏量於pH2.0噴施1週後迅速增高,顯示葉片細胞受到傷害。強酸使表層導度下降且無日變化,氣孔導度則各處理間並無差異。「臺農15號」葉片之可溶性蛋白質和葉綠素於pH2.0及3.0處理2週後開始下降,「高雄選10」號則祗有pH2.0處理有下降趨勢。

並列摘要


The primary objectives were to determine the acidic rain stress cause alternation in the soybean foliar injury responses. potted soybean (Glycine max.L. cv. ”Tainurig 15” and ”Kaohsiung select 10”) were exposed to two 1 mm simulated rain episodes per week at specific acidities from pH 2.0, 3.0, 4.0 and 5.6 treatments. Each treatment was exposed to 0.5hr spraying in the morning between 8:00 and 9:00 on consecutive days for 5 weeks after anthesis (R2) stage. Acid rain treatment at pH 2.0 induced the formation of pale brown necrotic spots on the leaves within 2 weeks after exposure. The electrolyte leakage of the first trifoliate leaf to acid rain of pH 2.0 for 1 week was significantly greater than that from the other treatments. cuticular stomata exposed to the acid rain of pH 2.0 showed a smaller aperature and did not follow diurnal changes. However, acidity of rain had no effect on stomatal conductance of the soybean. After 2 weeks of exposure, in pH 2.0 and pH 3.0 treatments, soluble protein and chlorophyll content of ”Tainung 15” showed a significant decrease. However, ”Kaohsiung selection 10” was not significantly affected by pH 3.0 episode.

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