Amylose content of milled rice is a significant quality parameter. Genetic analysis of amylose content was conducted using single-grain amylose analysis. Intermediate amylose content to low appeared to be controlled by a single incompletely dominant gene in the combinations between the intermediate and low amylose parents. Two incompletely dominant complementary genes conditioning high over low were suggested from the combinations between high and low amylose parents. A genetic hypothesis of a single incompletely dominant gene controlling high to intermediate amylose was fitted in mating high with intermediate amylose parents. Evidence that amylose content was also influenced by genes of minor effects, or modifiers was also observed in some combinations. The segregation model of endosperm characteristics was illustrated under the assumption of no dosage effect.