Maturation of Yellowfin Tuna(Thunnus Albacares)Ovaries from the Southern Waters of Taiwan

臺灣南部海域產黃鰭鮪卵巢的成熟

吳繼倫(Chi-Lun Wu) ； 郭慶老(Chin-Lau Kuo) ； 張正芳(Cheng-Fang Chang)

《臺灣水產學會刊》 24卷4期 (1997/12) Pp. 299-312

https://doi.org/10.29822/JFST.199712.0004

摘要

以JAK1起動子置於黃鰭鯛生長激素基因(ypGH)之前構建一新轉殖基因載體pJAKY，将此轉殖基因以電穿孔法帶入吳郭魚精子內，再以此經由電穿孔過攜有轉殖基因之精子，與成熟的吳郭魚卵授精，授精卵仍可正常孵化並發育。自授精起算第181天，仍有14尾稚魚存活。自其中13尾取胸鰭及腹鰭萃取DNA做聚核脢鏈索反應法(PCR)檢測，皆証實黃鰭鯛生長激素基因存在魚體細胞內。體型最小的一尾（11.72克）取肝、腦、肌肉及血液萃取RNA，並做反轉錄聚核脢鏈索反應法(RT-PCR)檢測，結果在肝細胞檢測出ypGH mRNA的存在，並在腦細胞檢測出小量ypGH mRNA的存在，顯示轉殖基因確有表現功能。但在血液及肌肉則無表現。在自授精起算第213天，再抽取體型最大的兩尾（74.79克及66.02克）基因轉殖吳郭魚血做反轉錄聚核脢鏈索反應法檢測，結果皆檢測出ypGH mRNA存在血液中。經越冬蓄養14個月後，測量體重做生長指標，以平均體重做比較，結果顯示基因轉殖吳郭魚較非基因轉殖吳郭魚成長快四個月。

關鍵字

黃鰭鮪 ； 成熟 ； 卵發生

並列摘要

An”all-fish”transgene,pJAKY was constructed by which consisted of JAK1promoter and yellowfin porgy growth hormone(ypGH).Tilapia sperm which electroporated with pJAKY was used as plasmids carrier for gene transfer.These electroporated-sperm were immediately mixed with eggs and subsequently added withwater to stimulate fertilization.At day 181 since fertilization,14 presumptivetransgenic fishes remained alive.The genomic DNA from 13 fishes of them wereextracted from their pectoral and anal fins for PCR analysis.Presence of the integrated transgene within the genome was confirmed by that the expressed ypGHPCR fragments were positively detected from all examined genomic DNA samples.Further,one out of 14 transgenic fish was sacrificed to monitor the expression ofthe transgene at the transcriptional level by RT-PCR analysis.The expression ofypGH mRNA in the tissue of liver and brain was surely present and could bedirectly visualized on the agarose gel after electrophoresis.But the ypGH mRNAwas absent in the tissue of muscle and blood.During the rearing period after 14months,the average body weight of those 13 presumptive transgenic fishes wasscored by 1.73 folds weightier than that of non-transgenic fishes.Using mean ofbody weight as parameter for comparison,the group of transgenic fish grow fasterthan the group of non-transgenic fish around 4 months.

並列關鍵字

Yellowfin Tuna ； Maturation ； Oogenesis