Fishing Ground Formation of Larval Anchovy in Relation to Oceanographic and Meteorological Conditions in the I-Lan Bay of Northeastern Taiwan

從吳郭魚（莫三鼻口孵魚）之腦下垂體抽取核醣核酸後，利用魚類生長激素專一性極高之引子，當反轉錄時作用之引子，利用聚合酶連鎖反應選殖出莫三鼻口孵魚之生長激素cDNA，莫三鼻口孵魚之生長激素由204個胺基酸所構成，其核苷酸序列與吳郭魚（尼羅口孵魚）大約具有98％之相似性。pQE30載體連接莫三鼻口孵魚之生長激素核苷酸(cDNA)序列，利用大腸桿菌系統表現出大約22 kDa之重組生長激素蛋白質。莫三鼻口孵魚之基因結構體由6個外顯子(intron)與5個內隱子(exon)所組成，與尼羅口孵魚之核苷酸序列相似性大約97％。

關鍵字

鳀科稚魚；漁場形成；衛星遙測；表水溫

並列摘要

The complementary DNA(cDNA)encoding the prepeptide growth hormone(pre-GH)from tilapia(Oreochromis mossambicus;om)has been cloned and sequenced.The cDNAwas derived by reverse transcription of RNA from total RNA of the pituitary gland using themethod of polymerase chain reaction(PCR)and oligonucleotide primers specific for fishgrowth hormone as amplification primers.The omGH cDNA encoded a pre-hormone of204 amino acid residues.The nucleotide sequence of omGH showed greater than 98%homology with that of Tilapia niloticus indicating a high degree of conservation for GHcDNAs sequence within the genus Tilapia.Mature omGH cDNA was inserted in anEscherichia coil expression vector(pQE30 vector,the pQE30 vector containing a 6×Hisaffinity tag),and a protein of about 22 kDa was expressed in E.coli cells.The bacteriallyexpressed omGH protein,contained within the inclusion body pellet,was dissolved in 8 Murea,and purified in a Ni-NTA column.Both the cloned tilapia(O.mossambicus)gene andthat of T.niloticusis were divided into 6 exons and 5 introns.Comparison of the omGHgenomic DNA sequence with that of Tilapia niloticus showed a homology of approximately97%.This is significant from an evolutionary standpoint,suggesting a high degree ofconservation for the GH gene within the genus Tilapia.

並列關鍵字

Larval Anchovy ； Fishing Ground Formation ； Satellite Remote Sensing ； Sea Surface Temperature

國際替代計量

Fishing Ground Formation of Larval Anchovy in Relation to Oceanographic and Meteorological Conditions in the I-Lan Bay of Northeastern Taiwan

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