The Application of the Taiwan Fleet Dynamics Management System to Fishing Fleets and Fishery Resources

漁船動態管理系統在漁船和漁業資源管理之應用

江福松(Fu-Sung Chiang) ； 孫金華(Chin-Hwa Sun)

《臺灣水產學會刊》 26卷2期 (1999/06) Pp. 103-114

https://doi.org/10.29822/JFST.199906.0005

摘要

本研究主要針對短期體外維持蝦血球細胞的培養條件進行改善，以利蝦子細胞性防禦機制的研究。來自淡水長腳大蝦(Macrobrachium rosenbergii)的血球樣本，分別培養在(1)MCHBSS培養基，於4℃下，30或60分鐘；(2)不同的培養基，於4℃下，30分鐘；及(3)L-15培養基，於4、25或30℃下，30分鐘。血球培養30分鐘後的活存率（53.3％）高於培養60分鐘（16.7％）者。使用不同培養基時，培養於L-15（63.5％）、L-15-胎牛血清（58.7％）、及L-15-蝦血清（55.7％）三種培養基的血球活存率無明顯差異；但以MCHBSS培養後，活存率明顯降低（36.8％）。當血球以L-15培養基於不同溫度下培養30分鐘後，培養在30℃的血球活存率（35.1％）明顯低於培養在4℃（56.1％）或25℃（52.6％）者。

關鍵字

漁船動態管理 ； 漁業資源管理 ； 中日暫定措施水域 ； 資訊系統

並列摘要

This study intended to improve the short-term in vitro maintenance of hemocytes inorder to study cellular defense mechanisms in prawn.Hemocyte samples from Macro-branchium rosenbergii were cultured in(1)modified complete Hank's balanced salt solution(MCHBSS)at 4℃ for 30 or 60 min,(2)different media at 4℃ for 30 minutes,and(3)L-15medium at 4,25 or 30℃ for 30 min,respectively.A higher percentage of hemocyte viabilitywas obtained following hemocytes cultured for 30 min(53.3%)compared to culturedfor 60 min(16.7%).Using different media,there were no significantly difference of hemocyteviability between L-15 medium(63.5%),L-15-FCS(58.7%)and L-15-PS(55.7%);however,a significant decrease of viability was obtained following hemocytes cultured inMCHBSS(36.8%).Following hemocytes incubated in L-15 for 30 min at different temperatures,the viability was significantly lower at 30℃(35.1%)than at 4℃(56.1%)or 25℃(52.6%).

並列關鍵字

Fleet Movement Dynamics Management ； Fisheries Resources Management ； Sino-Japan Co-Management Zone ； Information System