Effects of Ozone on GH1 and GH2 Genes Expression and Growth Enhancement Using Rainbow Trout

臭氧對虹鱒的GH1和GH2基因的表達和成長之影響

森司(Tsukasa Mori) ； 出口文絵(Fumie Deguchi) ； 李大雄(Miei Sakurai) ； 櫻井美榮(Sakae Fukunaga) ； 福永榮(Tai-Hung Lee)

《臺灣水產學會刊》 28卷4期 (2001/12) Pp. 269-274

https://doi.org/10.29822/JFST.200112.0007

摘要

The expressions of the GH1 and GH2 genes were examined by competitive RT-PCR using fry rainbow trout (Oncorhynchus mykiss) cultured with low concentration of ozonized water. λRNA having GH primers at both 5' and 3' sites of the gene was used for the competitive RT-PCR. The amount of GH1 gene expression treated with ozone was significantly (p＜0.05) greater than that of control fish after 3 month, however, there were no significant differences in GH1 and GH2 between ozone and control after 5 months culture. The ozone treatment has a tendency to stimulate growth enhancement, but there was no significant (p=0.05) differences between them.

關鍵字

GH基因表達 ； 虹鱒腦下垂體 ； 臭氧

並列摘要

The expressions of the GH1 and GH2 genes were examined by competitive RT-PCR using fry rainbow trout (Oncorhynchus mykiss) cultured with low concentration of ozonized water. λRNA having GH primers at both 5' and 3' sites of the gene was used for the competitive RT-PCR. The amount of GH1 gene expression treated with ozone was significantly (p＜0.05) greater than that of control fish after 3 month, however, there were no significant differences in GH1 and GH2 between ozone and control after 5 months culture. The ozone treatment has a tendency to stimulate growth enhancement, but there was no significant (p=0.05) differences between them.

並列關鍵字

GHs genes expression ； Rainbow trout pituitary ； Ozone