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The Acid-base Balance of the Hemolymph of the Hard Clam (Meretrix lusoria) is Affected by Aerial Exposure and Cellular Hypoxia-inducing Factors
曝氣和誘導細胞性缺氧因子影響文蛤(Meretrix lusoria)血淋巴酸鹼平衡之研究
摘要
文蛤在15℃時，經曝氣一分鐘後，其血淋巴的pO2由最初的23.8 mmHg降至18.3 mmHg，然而在30℃時，其血淋巴的pO2則由27 mmHg降至14 mmHg。文蛤經短暫的曝氣後，其血淋巴pO2的快速下降，可能是由於其血淋巴含有低濃度的氧氣攜帶者。真地，在文蛤血淋巴中測不到血紅素的存在，而且其血藍素的含量僅為0.075 mM。文蛤經曝露於無鎂的人工海水10小時後，其血淋巴的pO2顯著地由24 mmHg降至13 mmHg，然而其血淋巴的pCO2和HCO(下標 -)3，則分別由4.8 mmHg和4.3 mmol/l增加至10 mmHg和9.9 mmol/l，當曝氣時間延長為48 h時，則分別降至6.5 mmHg和7.6 mmol/l。在實驗期間，鉀離子能夠分別降低和增加血淋巴的pO2和pCO2，然而對血淋巴的pH或HCO(上標 -)3的含量卻沒有影響。KCN能夠持續地降低血淋巴的pO2，然而僅在暴露的早期能夠增加血淋巴的pH和HCO(上標 -)3的含量。
並列摘要
After 1 min aerial exposure, the hemolymph pO2 of hard clams (Meretrix lusoria) was reduced from 23.8 mmHg initially to 18.3 mmHg at 15℃, while it significantly decreased from 27 mmHg initially to 14 mmHg at 30℃. This rapid decline in the hemolymph pO2 of hard clams may have been due to a low concentration of oxygen carriers in the hemolymph. Indeed, the hemoglobin in hemolymph of hard clams was not detectable, while a value of 0.075 mM hemocyanin in the hemolymph was obtained. After clams were exposed to Mg(superscript +2)-free artificial seawater for 10 h, the hemolymph pO2 of significantly dropped from 24 to 13 mmHg. However, the hemolymph pCO2 and HCO(superscript -)3increased from 4.8 mmHg and 4.3 mmol/l initially to 10 mmHg and 9.9 mmol/l at 10 h of exposure and then decreased to 6.5 mmHg and 7.6 mmol/l at 48 h of exposure, respectively. Potassium reduced the hemolymph pO2 and increased the hemolymph pCO2, while it had no influence on hemolymph pH or HCO(superscript -)3 during the experiment. KCN persistently decreased the hemolymph pO2, while its only effect on the hemolymph pH and HCO(superscript -)3 occurred in the early phase of exposure.
延伸閱讀
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