- 期刊
- OpenAccess
The Inhibitory Effect of Anti-cancer Compounds Extracted from Marine Microalgae on the Growth of Cancer Cells
萃取自海洋微藻抗癌化合物抑制癌細胞生長的影響
摘要
Four species of marine microalgae, Isochrysis aff. galbana, Nannochloropsis oculata, Cheatoceros muelleri and Tetraselmis chui, common utilized in aquaculture were used as materials. The anti-cancer compounds of marine microalgae were extracted by Tris buffer, 27% ethanol, and 62% ethanol. The inhibitory abilities of anticancer compounds in Tris extract, 27% ethanol extract, and 62% ethanol extract on the growth of colon cancer (SW 480 and HT 29 cell lines) and gastric cancer (TMC-1 and TSGH9201 cell lines) were determined by MTT assays. The inhibitory ability of anti-cancer compounds extracted from I. aff. galbana on the growth of TMC-1 was found to be the strongest. Anti-cancer compounds of I. aff. galbana in Tris extract, 27% ethanol extract, and 62% ethanol extract had strong inhibition on the growth of these cell lines. The molecular size of anticancer compounds in Tris extract was found to be smaller than that of blue dextran after they were separated by Sephacryl S-200 column. Active fractions from Sephacryl S-200 column were collected and further separated by DEAE-Sepharose column. Active fractions were eluted by 0.25~0.5 M NaCl. Therefore, anti-cancer compounds should carry much negative charges. The further investigation on the study of characteristics of these compounds is needed.
並列摘要
以廣泛應用於水產養殖的四種海洋微藻(Isochrysis aff. galbana、Nannochloropsisoculata、Cheatoceros muelleri和Tetraselmis chui)作為萃取的材料，以Tris緩衝溶液、27%乙醇和62%乙醇萃取海洋微藻的抗癌化合物。以MTT測試法評估Tris萃取液、27%乙醇萃取液和62%乙醇萃取液中抗癌化合物對大腸癌細胞(SW 480和HT 29細胞株)和胃癌細胞(TMC-1和TSGH9201細胞株)抑制的影響。在四種海洋微藻中，萃取自等鞭金藻的抗癌化合物對TMC-1細胞株的抑制成長的影響最大。等鞭金藻Tris萃取液、27%乙醇萃取液和62%乙醇萃取液中抗癌化合物對上述四種癌細胞株的成長有很大的抑制作用，以Sephacryl S-200管柱分離Tris萃取液的抗癌化合物，發現其分子量小於blue dextran的分子量。取經Sephacryl S-200管柱分離的有效成分再經DEAESepharose管柱分離，可以0.25~0.5 M NaCl的梯度將管柱中的有效成分洗出，顯示抗癌化合物應該攜帶高密度的負電荷。有關這些抗癌化合物的分子特色仍不清楚，有待進一步探討。
延伸閱讀
- 徐慧君（2006）。海洋聚球藻藻藍素的免疫抗癌及抗氧化活性〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2006.10502
- 劉琳、馬超、姚菊明（2015）。可吸收抗菌海藻纖維的製備及其性能研究。華岡紡織期刊，22(3)，147-151。https://www.airitilibrary.com/Article/Detail?DocID=10259678-201509-201701160013-201701160013-147-151
- 李惠婷（2014）。探討褐藻小海帶抑制人類癌細胞生長〔博士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2014.01122
- Lee, H. T., & Huang, R. (2012). 海藻Petalonia binghamiae褐藻酸鈉抑制癌細胞生長的活性. 臺灣水產學會刊, 39(4), 257-267. https://doi.org/10.29822/JFST.201212.0005
- 吳宗孟、朱建宏、葉信平、邱秋霞、吳育昇（2019）。海藻抗發炎活性物質萃取及其機能性應用。屏科大研發專刊，()，10-10。https://www.airitilibrary.com/Article/Detail?DocID=P20110422001-201912-202105210010-202105210010-10-10-042