Dietary Nucleotides Supplementation Enhances Growth, Immune Responses and Survival to Vibrio alginolyticus of Juvenile White Shrimp, Litopenaeus vannamei

飼料中添加核苷酸可提高南美白對蝦之成長、免疫反應及Vibrio alginolyticus感染後之存活率

蕭錫延(Shi-Yen Shiau) ； 林鈺鴻(Yu-Hung Lin)

《臺灣水產學會刊》 42卷3期 (2015/09) Pp. 179-187

https://doi.org/10.29822/JFST.201509_42(3).0005

摘要

The study was to investigate the effect of dietary nucleotides (NT) on growth, immune responses and survival to Vibrio alginolyticus in Pacific white shrimp, Litopenaeus vannamei. Nucleotides was added to basal diet at 0, 60, 120 and 240 mg NT/kg diet for a total of 4 experimental diets. Each diet was fed to four replicate groups of shrimp (initial body weight: 1.69 ± 0.02 g) in a recirculated rearing system for 8 weeks. Final weight, weight gain and granular cells count were significantly higher (P ＜ 0.05) in shrimp fed diets with ≥ 60 mg NT/kg diet than that in shrimp fed the NT-free control diet. Total hemocyte and semi-granular cells counts were the highest in shrimp fed the diet with 240 mg NT/kg diet, followed by shrimp fed the diet with 60 mg NT/kg diet, and the lowest in shrimp fed the control diet. Respiratory burst (O_2^- production ratio) activity was higher in shrimp fed diets with 120 and 240 mg NT/kg diet than that in shrimp fed the control diet. Phenoloxidase (PO) activity was higher in shrimp fed diets with 60-120 mg NT/kg diet than that in shrimp fed the control diet. After injection with Vibrio alginolyticus for a week, shrimp fed the diet with 60-240 mg NT/kg diet had higher survival than shrimp fed the control diet. These results suggest that nucleotides supplemented at 60-120 mg NT/kg in diet are adequate for enhancing growth, immune responses and survival to V. alginolyticus of Pacific white shrimp.

關鍵字

Pacific white shrimp ； Nucleotides ； Growth ； Immune responses ； Disease resistance

並列摘要

本研究為探討飼料中添加核酸(nucleotides, NT)對南美白對蝦成長、免疫反應及Vibrio alginolyticus感染後存活率之影響。基礎飼料中分別添加0、60、120及240 mgNT/kg diet之核酸，實驗飼料共分為四組，分別餵予初重1.69 ± 0.02 g之白蝦，每組飼料四重覆，於循環水系統中飼養八週，餵食實驗結束後進行V. alginolyticus之攻擊試驗。白蝦餵食≥ 60 mg NT/kg diet飼料其末重、增重率與顆粒細胞計數顯著高於(P ＜0.05)未添加核酸之控制組。白蝦之總血球與半顆粒細胞計數以餵食240 mg NT/kgdiet飼料組最高，其次為60 mg NT/kg diet組，以控制組最低。白蝦血淋巴液呼吸爆發活性以餵食120-240 mg NT/kg diet飼料組高於控制組。酚氧化酉每 活性以餵食60-120mg NT/kg diet飼料組高於控制組。白蝦經V. alginolyticus感染後一週，存活率以餵食60-240 mg NT/kg diet飼料組高於控制組。研究結果顯示，飼料中添加60-120 mg NT/kg diet核酸即可有效提升白蝦之成長、免疫反應及V. alginolyticus感染後之存活率。

並列關鍵字

南美白對蝦 ； 核苷酸 ； 成長 ； 免疫反應 ； 疾病抵抗能力