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An Increase in Immune Response and Protection against Vibrio Alginolyticus in the White Shrimp Litopenaeus Vannamei after a Fucoidan Immersion
浸泡褐藻醣膠白蝦免疫力增加及具對Vibrio Alginolyticus抵抗
摘要
白蝦浸泡於0、100、200及400 mg/l褐藻醣膠的海水，經5小時後，以溶藻弧菌（6 ×106 CFU／蝦）進行攻擊實驗，放回乾淨的海水中。發現浸泡於褐藻醣膠的白蝦，感染弧菌48-120小時後，其存活率皆高於對照組。另外，白蝦浸泡於0、100、200及400 mg/l褐藻醣膠，經1、3及5小時後，分析血球數、酚氧化酵素活性、呼吸爆、超氧歧化酵素活性、穀胱甘肽過氧化氫酵素活性及對溶藻弧菌的吞噬及清除能力。結果顯示，這些免疫因子隨著褐藻醣膠濃度及時間增加而增加。浸泡於含褐藻醣膠100、200及400 mg/l白蝦，對溶藻弧菌的吞噬活力及清除能力增加。綜合上述，白蝦浸泡於含褐藻醣膠100 mg/l具提昇免疫力，以及對溶藻弧菌感染的保護。
並列摘要
White shrimp Litopenaeus vannamei that immersed in seawater containing fucoidan at 0 (control), 100, 200 and 400 mg/l after 5 h were challenged with Vibrio alginolyticus at 6 x 106 colony-forming units (cfu)/shrimp, and then placed in seawater. The survival rate of shrimp that immersed in seawater containing fucoidan at either dose was significantly higher than that of control shrimp over 48~120 h. In another experiment, hemocyte count, phenoloxidase (PO) activity, respiratory burst (RB), superoxide dismutase (SOD) activity, and glutathione peroxidase (GPx) activity, phagocytic activity and clearance efficiency against V. alginolyticus were examined when the white shrimp L. vannamei were immersed in seawater containing different concentrations of fucoidan (0, 100, 200, and 400 mg/l) for 1, 3, and 5 h. These immune parameters increased directly with the concentration of fucoidan and exposure time. White shrimp L. vannamei that immersed in seawater containing fucoidan at 100, 200, and 400 mg/l had increased phagocytic activity and clearance efficiency against V. alginolyticus. It was concluded that the shrimp immersed in seawater containing fucoidan at a concentration of 100 mg/l exhibited an immunomodulatory effect, as well as protection against V. alginolyticus infection.
延伸閱讀
- Chang, C. C., Toscano, F. S. M., & Cheng, W. (2013). 飼料添加粉葉馬尾藻酸性溶液萃取物增強草蝦免疫反應及抗病能力. 臺灣水產學會刊, 40(1), 57-70. https://doi.org/10.29822/JFST.201303_40(1).0005
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- Chang, C. C., Gao, L. H., & Cheng, W. (2013). 飼料添加重緣葉馬尾藻熱水萃取物對白蝦免疫及抗溶藻弧菌能力之影響. 臺灣水產學會刊, 40(1), 27-41. https://doi.org/10.29822/JFST.201303_40(1).0003
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