Effect of Dietary Administration of Hot-water Extracts of Sargassum cristaefolium on the Immunity of White Shrimp Litopenaeus vannamei and Its Resistance Against Vibrio alginolyticus

飼料添加重緣葉馬尾藻熱水萃取物對白蝦免疫及抗溶藻弧菌能力之影響

張欽泉(Chin-Chyuan Chang) ； 高良華(Liang-Hua Gao) ； 鄭文騰(Winton Cheng)

《臺灣水產學會刊》 40卷1期 (2013/03) Pp. 27-41

https://doi.org/10.29822/JFST.201303_40(1).0003

摘要

以添加重緣葉馬尾藻（Sargassum cristaefoliu）熱水萃取物0、0.5、1及2 g/kg之飼料餵飼白蝦（Litopenaeus vannamei）0、3、6、9和18天後，評估其對總血球、酚氧化酵素活性、超氧離子含量、超氧歧化酵素、以及吞噬、清除能力之影響。並進行白蝦餵飼重緣葉馬尾藻熱水萃取物0、0.5、1及2 g/kg之飼料18天後對對溶藻弧菌感染之感受性試驗。結果顯示，白蝦餵飼含重緣葉馬尾藻熱水萃取物2 g/kg之飼料，其酚氧化酵素活性、超氧陰離子以及吞噬、清除能力皆顯著提升且提高對溶藻弧菌感染之存活率。使用重緣葉馬尾藻熱水萃取物，以2 g/kg濃度添加於飼料中可誘發白蝦免疫調節及增強免疫能力、增加其對溶藻弧菌感染之抵抗力。

關鍵字

白蝦 ； 熱水萃取 ； 重緣葉馬尾藻 ； 免疫調節 ； 感受性

並列摘要

The total haemocyte counts, phenoloxidase (PO) activity, respiratory bursts, superoxide dismutase (SOD) activity, and phagocytic activity and clearance efficiency of Litopenaeus vannamei fed hot-water extract of Sargassum cristaefolium (SCE) at 0 (control), 0.5, 1 and 2 g/kg were evaluated after feeding for 0, 3, 6, 9, and 18 days. The susceptibility of L. vannamei fed diets containing SCE at 0 (control), 0.5, 1 and 2 g/kg for 18 days and then challenged with Vibrio alginolyticus was also conducted. The results indicated that PO activity, respiratory burst, and SOD activity, and phagocytic activity and clearance efficiency to V. alginolyticus, and the survival rate after challenging with V. alginolyticus all significantly increased when the shrimp were fed SCE-containing diets at 2 g/kg. It was concluded that administration of hot water extract of S. cristaefolium in the diet at 2 g/kg induced immune modulation and enhanced the immune ability of L. vannamei, and increased resistance to V. alginolyticus infection.

並列關鍵字

Litopenaeus vannamei ； hot water extract ； Sargassum cristaefolium ； immune modulation ； susceptibility