Effect of Monascus on immune and physiological responses of Pacific white shrimp (Litopenaeus vannamei)

Ya-Ting Chen ； Chih-Chung Wu ； Chun-Hung Liu ； Jyh-Jye Wang ； Shu-Ling Hsieh

《臺灣水產學會刊》 45卷2期 (2018/06) Pp. 81-92

https://doi.org/10.29822/JFST.201806_45(2).0004

摘要

Monascus species belong to the fungi ascomycetes and in particular to the family of Monascaceae. Monascus have a variety of biological activities beneficial for human health. To investigate the regulatory roles of Monascus in physiological and immune responses in shrimp, we examined innate non-specific immune responses (including total hemocyte count (THC), respiratory bursts (RB), and phenoloxidase activity (PO) activity), physiological responses (including haemolymph glucose, triglyceride, lactate, and serum protein), and gene expressions (including lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP), superoxide dismutase (SOD), prophenoloxidase (proPO), ferritin (ferritin), and peroxinectin (PE) mRNA transcripts) of immersed Monascus at 0, 250, 500, and 1000 mg/L in Pacific white shrimp Litopenaeus vannamei. The results indicated Pacific white shrimp immersed Monascus in 500 mg/L can maintained lower glucose, lactate, and triglyceride levels at 0~48 hr. Immune responses significantly increased by THC, RB, and PO activity in L. vannamei. Especially immersed Monascus in 500 mg/L at 48 hr. Moreover, the gene expressions of LGBP, SOD, proPO, PE, and ferritin mRNA transcripts was significantly increased by Monascus. Especially immersed Monascus in 500 mg/L at 24 hr. This study suggests that immersed Monascus in 500 mg/L at 48 hr can enhance immunity and not change physiological homeostasis in L. vannamei.

關鍵字

Monascus ； Pacific white shrimp ； Immune ； Physiological