Large-scale screening and validation of molecular markers for the genetic assessment and management of Orbicular batfish (Platax orbicularis)

Yu-Ting Chiu ； Cheng-Hsin Liao ； Te-Hua Hsu ； Chang-Wen Huang ； Hung-Tai Lee

《臺灣水產學會刊》 47卷4期 (2020/12) Pp. 212-220

https://doi.org/10.29822/JFST.202012_47(4).0003

摘要

The genetic analysis of hatchery produced species has been increasingly important for stock enhancement since the loss of genetic diversity could potentially reduce the sustainability of stocked population. Orbicular batfish (Platax orbicularis) is a novel aquaculture species for stock enhancement in Taiwan. However, little is known for the genetic information of this species. In the present study, we aimed to develop molecular markers for the genetic analysis of this species. A total of 517 Simple sequence repeats (SSRs) were firstly identified based on in silico analysis of two public genomic data sets. 24 polymorphic SSRs were further selected and validated using multiplex PCR and high throughput sequencing (Illumina platform). We eventually found 7 highly polymorphic SSRs ideal for the genetic assessment and management of P. orbicularis.

關鍵字

Stock enhancement ； multiplex PCR ； next generation sequencing (NGS) ； genetic diversity

參考文獻

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