To attain superior hardenability of steel without further addition of alloy, austenite grain sizes should normally be controlled in the range of ASTM #2~5 for low/medium carbon wire rod. The cases were found that austenite grain size had fine grain of ASTM#6~9 in product. Based on the effect of AI, Nb, Ti elements on the fine -tuning technology of austenite grain size and the pinning effect of second- phase precipitates or inclusions on grain boundary movements, this study has examined the influence of cleanness in steel on grain refining process. The forward tracking experiments was performed to evaluate the correlation between grain size and inclusion. The results showed that the poorer quality of inclusion, providing more second-phase particles to inhibit grain growth. The first bloom in casting process should be cautiously controlled.