台灣本土微生物脫色性能與微生物燃料電池之可行性研究

Feasibility Study of Reductive Decolorization and Bioelectricity Generation Using Taiwan's Indigenous Microorganisms

藍士豪(S. H. Lan) ； 游立萍(Y. L. Ping) ； 呂惠慈(H. T. Liu) ； 陳博彥(B. Y. Chen)

《鑛冶：中國鑛冶工程學會會刊》 55卷4期 (2011/12) Pp. 107-114

https://doi.org/10.30069/MM.201112.0013

摘要

基於台灣永續發展及本土微生物資源開發之考量，本研究自溪頭、玉山地區之水樣和土樣中，利用Orange I（O I）及Orange II（O II）偶氮染料作為菌種篩選條件下，以此兩種染料分別對C644、C741、C760、U829用比脫色速率分析進行優異脫色菌株之排序評比，並經由分離純化及16S rRNA親緣樹分析菌種鑑定為γ-Proteobacteria之Aeromonas tecta C644及Aeromonas salmonicida C741、C760、U829，並將O I及O II染料脫色後之產物經GC／MS鑑定發現應為芳香胺中間物，因此確定染料脫色乃為非礦化作用之還原作用。後續將脫色較優異之菌株置入微生物燃料電池中以較易於脫色之Orange I染料及外電阻1KW操作條件下馴養約經30天，當達到穩定產電後將對各菌株做同時產電及脫色之評比分析，最後將本次結果於先前研究比較並分析混菌及純菌染料脫色與電壓產出之差異，以評估後續資源可利用之可行性。

關鍵字

永續發展 ； 染料脫色 ； 微生物燃料電池 ； 本土微生物資源

並列摘要

To explore a sustainable development of Taiwan's indigenous bioresources, this study selected soil and water samples from Sitou and Yushan for isolation of bacterial decolorizers using Orange I and Orange II as probing dyes. A comparative assessment of decolorization performance and 16S rRNA phylogenetic tree analysis indicated that the predominant decolorizers were all Gamma-Proteobacteria Aeromonas tecta C644, A. salmonicida C741, C760, U829. GC/MS identification of decolorized intermediates of O I and O II indicated that they were aromatic amines. It confirmed that color removal was reductive decolorization. To recycle and reuse of energy resources form wastewater treatment, we evaluated the bioelectricity generation capabilities of these decolorizer strains. We also assessed the feasibility of simultaneous bioelectricity generation and color removal of decolorizers for possible industrial applications.

並列關鍵字

Sustainable development ； Dye Decolorization ； Microbial fuel cells ； Indigeneus microbial resources