The effect of activated charcoal, sucrose and mineral concentration on send germination and needling growth of Pleione formosana were studied. it was shown that medium containing 2g/1 activated charcoal enhanced seedling growth and development obviously. The needs germinated in Knudson C medium with 30g/l suerose had better growth than those lower (10-20g/l) or higher (40g/l) concentration. The half-strength MS medium wan the most suitable for seedling growth among whole strength and 1/4MS or Knudson C medium.