臺灣一葉蘭之微體繁殖

Micropropagation of Pleione formosana

莊馥綱(Fu-Kang Chuang)

指導教授 ： 張祖亮

國立臺灣大學/生物資源暨農學院/園藝學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.01901

摘要

由臺灣一葉蘭腋芽再生之芽體及小植株繼代培養於1/3MS固體培養基及添加NAA及kinetin之1/2MS液體培養基。1號選系及2號選系之新生小芽叢繼代後持續增殖，但同為叢生芽之8號選系並未顯著增殖。1號選系以完整的小植株進行培養，僅萌發頂芽及腋芽，混合系之假球莖縱切後，則有叢生芽形成。在單株培養試驗中，完整的小植株在添加NAA及kinetin之1/2MS固培養基亦無法形成叢生芽。在薄片培養試驗中，假球莖縱切為薄片後，置於不含植物生長調節劑之1/3MS培養基，約在培養後6-7週可見叢生芽產生，平均每球可得12芽。但橫切處理者，大部份培植體僅長出1-2芽。添加2,4-D可誘導培植體直接形成癒傷組織，或使發育中的頂芽及腋芽之基部形成癒傷組織。在根尖培養試驗中，取自1號選系之根培植體，在NAA濃度0.1-.0.2 mg./l時，可形成黃色鬆軟之癒傷組織，在NAA濃度0.5-.2 mg./l時，則形成綠色緊實之癒傷組織。癒傷組織繼代培養於1/3MS固體培養基，僅產生不定根，大部份癒傷組織在繼代後2個月褐化死亡。

關鍵字

癒傷組織 ； 薄片培養 ； 臺灣一葉蘭 ； 微體繁殖

並列摘要

Shoots and plantlets regenerated from axillary buds of Pleione formosana were subcultured in 1/3MS solid medium and 1/2MS liquid medium supplemented with kinetin and NAA. Nascent multiple shoots of MF#1M and MF#2 kept proliferation after subculture, but MF#8, another line producing multiple shoots, did not show significant proliferation. Intact plantlet of MF#1S only sprouted tip bud and lateral bud, but pseudobulb of MIX cut longitudinally developed multiple shoots on each explant. In single-plantlet experiment, intact plantlet also failed to form multiple shoots in 1/3MS solid medium supplemented with NAA and kinetin. In thin section culture experiment, multiple shoots were visible on explants obtained by longitudinal section after 6-7 weeks of culture in 1/3MS solid medium free of plant growth regulator with 12 shoots per bulb on average. However, most explants only developed one or two shoots by transverse thin section. Application of 2,4-D induced callus formation on explants or on the base of developing lateral buds and tip buds. In root tip culture experiment, root tips from MF#1M formed yellow, soft callus at NAA concentration 0.1-0.2 mg/l and green, compact callus at NAA concentration 0.5-2 mg/l. Only adventitious root developed after subculture of callus in 1/3 MS medium and most turned brown after 2 months after subculture.

並列關鍵字

callus ； thin section culture ； micropropagation ； Pleione formosana

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