摘要
本試驗以黃紋萬年麻花莖上之小鱗莖頂芽及鱗片爲培植體，置於MS基本培養基進行初代培養。經12週初代培養，頂芽培植體形成芽體百分率達100%，顯著高於鱗片培植體；而平均每個培植體産生之芽體數，則以培養基含1mg/l NAA及4mg/l BA的3.8個最高，其次爲2mg/l BA之3.0個。由鱗片培植體誘致之芽體，則易産生不具斑紋之綠葉株。繼代培養以MS培養基含1mg/l NAA及2mg/l TDZ處理，對芽體增殖效果最佳。瓶苗移植出瓶，以株高4-8 cm植株出瓶成活率高且相對生長速率最高。而瓶外生根則以1000mg/l NAA粉衣處理對發根效果最佳。
並列摘要
The apices or scales from the bulbils of Furcraea foetida cv. Striata were used as the explants for in vitro culture. Results indicated that the efficiency of shoot formation from bulbil-apex explants was higher than that from scale explants. An average of 3.8 shoots was formed from each bulbil-apex explant cultured in the MS medium supplemented with NAA 1mg/l and BA 4mg/l. In comparision, an average of 3.0 shoots was formed in the MS medium supplemented with BA 2 mg/l, after twelve weeks inoculation. The homogeneous green plantlets frequently regenerated from striated-scale explants. The optimum timing for subculture was 5-6 weeks after inoculation and shoots proliferation was significantly enhanced in the MS medium supplemented with NAA 1 mg/l and thidiazuron (TDZ) 2 mg/l. The optimum height for plantlets transferred from the test tube to soulless mixes was 4-8 cm, which resulted in high survival rate and high relative growth rate. For root formation in vivo, plantlets treated with NAA 1000 mg/l was found to have the best result.
延伸閱讀
- 陳俊仁、胡文若、孫文章、王瑞章（2005）。斑葉月桃之組織培養。臺南區農業專訊，(52)，1-4。https://doi.org/10.29557/YYWYLL.200506.0001
- 季景元、周學淳、李雨霖（1963）。黃麻扦插採種法之研究。中華農業研究，12(1)，1-34。https://doi.org/10.29951/JARC.196303.0001
- 葉志新（2014）。天麻栽培與利用。桃園區農情月刊，(182)，4-4。https://www.airitilibrary.com/Article/Detail?DocID=P20131017004-201409-201411060004-201411060004-4-4
- 林婉玉、葉茂生（2009）。In Vitro Seed Germination and Development of Nervilia Plicata(Andr.) Schltr。植物種苗，11(1)，41-56。https://doi.org/10.30077/SN.200903.0003
- Cavalcante, M. Z. B., Amaral, G. C., Avelino, R. C., Brito, L. P. D. S., & Cavalcante, Í. H. L. (2014). Propagation of Alternanthera dentata through cutting process. Comunicata Scientiae, 5(2), 170-177. https://www.airitilibrary.com/Article/Detail?DocID=21775133-201406-201605200020-201605200020-170-177