紫花脈葉蘭無菌發芽及發育的研究

In Vitro Seed Germination and Development of Nervilia Plicata(Andr.) Schltr

林婉玉(Wan-Yu Lin) ； 葉茂生(Mau-Shing Yeh)

《植物種苗》 11卷1期 (2009/03) Pp. 41-56

https://doi.org/10.30077/SN.200903.0003

摘要

本研究目的在探討紫花脈葉蘭無菌播種發芽的適合條件及發芽形態之觀察，以建立種子發芽繁殖技術。紫花脈葉蘭經人工授粉後，初期蒴果迅速膨大，之後生長漸趨緩慢，大致呈拋物線曲線生長。取授粉後15天之蒴果進行無菌播種於1/2 MS添加0.5 g/L peptone 及1 g/L Hyponex No.1培養基黑暗處理90天，其發芽率較高（59.62 %），繼代培養基270天球莖及芽體形成率分則為70.7﹪及57％時，種子萌芽後根莖及幼苗生長良好。

關鍵字

紫花脈葉蘭 ； 無菌發芽 ； 發育

並列摘要

The main objectives of this study found out the suitable condition for in vitro germination of seeds, and observed the development this endangered orchid species, Nervilia plicata. The growth of capsules of N. plicata was fast in the initial phase. The expansion curve seemed parabolic. The capsules harvested at 15 days after pollination were used for the seed asymbiotic germination. Seeds were sowed in a 1/2MS medium containing with 0.5 g/L peptone and 1 g/L Hyponex #1 had the best germination (59.62%) after 90 days dark culture, and with well grown rhizomes and shoot elongation after 270 days cultured.

並列關鍵字

Nervilia plicata ； In vitro seed germination ； Development