綬草無菌播種與生長發育

In vitro Seed Germination and Plant Development of Spiranthes sinensis (Pers.) Ames

張正(Chen Chang) ； 陳盈君(Yin-Chun Chen) ； 嚴新富(Hsin-Fu Yen)

《中國園藝》 49卷4期 (2003/12) Pp. 407-410

https://doi.org/10.6964/JCSHS.200312.0407

摘要

綬草(Spiranthes sinensis (Pers.) Ames)蒴果消毒後，將種子播在添加20g/l蔗糖、50g/l香蕉泥、1g/l活性碳的1/4MS基礎培養基，一個月後，形成原球體。培養五個月後，原球體長成葉長約2公分、根2-3條的幼苗，但其中有13.4%爲白化苗。將綠葉幼苗繼代到添加20g/l蔗糖、30g/l馬鈴薯泥、1g/l活性碳、150ml/l椰子水的1/4MS基礎培養基，再經二個半月後，幼苗可長到5公分長。幼苗經馴化後移植出瓶，97%的小苗可存活。且在四個月後，65%小苗抽苔開花，花色如同母株爲粉紅色，但有2.5%爲白花植株。此法可在一年之內完成綬草從種子發芽到成苗開花的生活史。

關鍵字

綬草 ； 種子發芽 ； 生長發育 ； 開花

並列摘要

The capsules of Spiranthes sinensis (Pers.) Ames. were sterilized by diluted sodium hypochlorite solution and the seeds were sown in the medium containing 1/4 strength of MS basal medium supplemented with 20g/l sucrose, 50g/l banana pulp and 1g/l activated charcoal. Green protocorms were then observed after one month. The seedlings were grown to plantlets with shoot and root after 5 months of culture. The plantlets were then subcultured onto the medium containing 1/4 strength of MS salt basal medium, 20g/l sucrose, 30g/l potato pulp, 1g/l activated charcoal and 150ml/l coconut milk. After 75 days of culture, the seedling (about 5 cm in height) were transplanted into a greenhouse with 97% survival rate. After 4 months of growing, 65% acclimatized plantlets flowered. The complete procedure of propagation the spiranthes orchids took less one year from seed germination to flowering.

並列關鍵字

Spiranthes sinensis Pers. Ames ； seed germination ； development ； flowering