花生花藥培養的研究Ⅲ.ABA及不同種類澱粉對花藥癒合組織分化之影響

Studies on the Anther Culture of Peanut Ⅲ. Effects of ABA and Starch on Differentiation of Anther-derived Calli

曾銀位(Yin-Wei Tseng) ； 葉茂生(Mau-Shing Yeh)

《農林學報》 49卷3期 (2000/09) Pp. 25-38

https://doi.org/10.30089/JAF.200009.0003

摘要

用栽培種花生(Arachis hypogaea)台南11號(TN11)、台農5號(TN5)及VR34等三個品種單核期花藥，由D2培養基誘導之癒合組織為培植體，探討不同濃度ABA及不同種類澱粉等處理對花生花藥癒合組織分化之影響，以期建立栽培種花生花藥培養之技術，進而作為花生育種之利用。其結果如下：
三品種之癒合組織在所有分化培養基培養後均無體胚或芽體之分化。但TN5以H1-A1、H1-A2培養基（MS+1mg/l NAA+2mg/BA和分別添加0.5、1mgl/ABA）與VR34以H1-A1培養基培養一個月後，轉移至MS培養基可誘導根的分化以ABA處理的癒合組織呈黃化膨鬆狀態，H1-A1較H1-A2培養基可維持較長時期的綠化，而H1-A2早期即已呈現褐化。
TN5、VR34二品種以H1-W、H1-C培養基（MS+1mg/l NAA+2mg/l BA和分別添加120g/l小麥、玉米澱粉）培養一個月後轉移至MS培養基可誘導根的分化。水稻澱粉使癒合組織呈鬆軟、多水、黃化狀態，培養效果不佳。

關鍵字

花生 ； 花藥培養 ； 離層酸 ； 澱粉 ； 分化

並列摘要

In order to establish the protocol for anther culture of peanut plant, the experiment was conducted to investigate the effects of ABA and starch on induction and differentiation ability of anther-derived callus. Calli inducted from anthers at uninucleate stage of the three peanut cultivars, TN1 1, TNS, and VR34 by D2 medium were used as materials. The results were summerized as follows:
After one month of culture, the calli of TN5 cultured on medium H1-A1 (MS+ 1mg/l NAA+2mg/l BA and 0.5mg/l ABA) and H1-A2 (MS+ 1mg/l NAA+2mg/l BA and 1mg/l ABA) and VR34 on medium H1-A1 were transferred to MS medium and the roots were differentiated. The calli treated with ABA were yellow and friable. The calli cultured on H1-A1 medium keep green longer than on H1-A2 medium. However the calli on H1-A1 medium turned brown very early.
After one month culture, TN5 and VR34 cultured on HI-W (MS+ 1mg/l NAA+2mg/l BA and 120g/l wheat starch) and H1-C media (MS + 1mg/l NAA+2mg/l BA and l20g/l corn starch) were transferred to MS medium could induce root differentiation. Subsequently, the calli induced on media with rice starch were pliable, succulent and yellow.

並列關鍵字

Arachis hypogaea peanut ； Anther culture ； Abscisic acid ABA ； Starch ； Differentiation