利用蛋白質體學方法研究UV-C照射對綠豆生長之影響

The Proteomic Study of the Ultraviolet-C Irradiation Effect for Vigna radiata

盧奕珊(Yi-Shan Lu) ； 楊明慧(Ming-Hui Yang) ； 呂濟宇(Chi-Yu Lu) ； 蔡婉琪(Wan-Chi Tsai) ； 田育彰(Yu-Chang Tyan) ； 許幼青(Yu-Ching Hsu) ； 郭淨紋(Ching-Wen Kuo) ； 劉育君(Yu-Chun Liu)

《農林學報》 62卷1期 (2013/03) Pp. 65-78

https://doi.org/10.30089/JAF.201303_62(1).0005

摘要

紫外光（UV-C）存在於太陽輻射中為高能量非游離輻射，為植物生長環境中不可避免之逆境。本研究以UV-C照射綠豆、並添加外源性抗氧化劑維他命C，觀察UV-C照射之綠豆其植株生長情形、脂質過氧化、類黃酮化合物含量及自由基清除能力。利用蛋白質體學研究法探討綠豆接受UV-C照射後其蛋白質變化情形，比對植物蛋白質序列資料庫後，發現UVC照射傷害可能誘導不同蛋白質表現，以此探討非游離輻射傷害對植物蛋白質之影響。結果顯示UV-C照射種子其植株發育不佳、脂質過氧化情形較低、類黃酮化合物含量提高，自由基清除能力下降，添加外源性維他命C則可促進側根生成；由差異性蛋白質比較中發現3個與UV-C傷害相關蛋白質、5個與添加外源性維他命C相關蛋白質，這些蛋白質可能參與植物生長發育調控與代謝並對抗UV-C 對植物生長的影響。

關鍵字

蛋白質體 ； 紫外線 ； 綠豆

並列摘要

Ultraviolet-C (UV-C) is high-energy non-ionized radiation in solar. Plants can not avoid UV-C irradiation in the environment. In this study, the Vigna radiata was exposured to UV-C irradiation, and applied with exogenous Vitamin C. The concentrations of malondialdehyde, flavonoids and the DPPH radical scavenging activity were measured by ELISA. To enhance our understanding the of Vigna radiata proteome, the proteins were analyzed using proteomic approaches followed by peptide fragmentation patterning. The experimental results showed that exogenous Vitamin C was induced the lateral root development. After exposure to UV-C irradiation, the concentration of malondialdehyde and the DPPH radical scavenging activity were down-regulated. The concentration of flavonoids was up-regulated. In this study, three proteins related to non-ionized radiation damage and five proteins related to apply exogenous Vitamin C were identified. Those proteins may affect the plant growth and development metabolism.

並列關鍵字

Proteomics ； Ultraviolet-C ； Vigna radiata