固態發酵處理對提升中藥渣抑菌活性及多酚物質含量之影響

Effects of Solid State Fermentation Treatment on Enhancing Antimicrobial Activity and Polyphenolic Substance Content of Chinese Herbal Medicinal Residues

温譽鈴(Yu-Ling Wen) ； 陳錦樹(Chin-Shuh Chen)

《農林學報》 62卷2期 (2013/06) Pp. 93-110

https://doi.org/10.30089/JAF.201306_62(2).0001

摘要

本研究以晉耆、合歡皮、刺五加、木賊、夜交藤、竹茹、柴胡、厚朴、穿心蓮、金銀花、一條根及括樓仁共12種中藥經熱水萃取後，剩餘藥渣為基本發酵基質，分別接種Aspergillus niger BCRC 32073或Aspergillus oryzae NCH 42進行固態發酵，探討發酵處理對提升中藥渣之抑菌活性並促進代謝產物生產之可行性，期能有助於提升中藥萃取廢棄物（藥渣）之附加價值。中藥渣分別以4種不同培養基組成調整，接種試驗菌株後於30℃下培養10天。結果顯示經A. niger BCRC 32073發酵後之合歡皮與穿心蓮藥渣之原兒茶酸（protocatechuic acid）含量明顯提升，刺五加和括樓仁則出現發酵前未含之成分。而經A. oryzae NCH 42發酵後之金銀花與括樓仁藥渣則產生微量紫丁香酸（syringic acid）及表兒茶素沒食子酸酯（epicatechin gallate，ECG）；此外，部份中藥渣經發酵後其抑菌範圍增加，其中以木賊、夜交藤、金銀花以及括樓仁之抑菌效果較為顯著。以上結果顯示經A. niger BCRC 32073或A. oryzae NCH 42固態發酵處理可提升部分中藥渣抑菌活性，提高中藥渣之附加價值。

關鍵字

中藥渣 ； 固態發酵 ； 抑菌活性

並列摘要

The feasibility of using solid state fermentation (SSF) treatment for increasing the utilization of extraction residues of Chinese herbal medicines (CHMs) was evaluated in this study. Twelve kinds of CHMs, including Hedysarum polybotrys Hand.-Mazz., Albizziae Cortex, Eleutherococcus senticosus, Equisetum hiemale L., Polygonum multiflorum Thunb., Bambusae Caulis in Taeniis., Bupleurum falcatum L., Magnolia officinalis, Andrographis paniculata, Lonicera japonica Thunb., Glycine tomentella Hayata and Trichosanthes kirilowii Maxim were used. Their aqueous extraction residues (CHMRs) were inoculated with a fungal strain, either Aspergillus orgzae NCH 42 or Aspergillus niger BCRC 32073, and SSF was performed at 30°C for 10 days. The SSF treatments were performed by dividing CHMRs into four groups based upon with or without nutrient supplement and inoculation. The antimicrobial activity and phenolic substances after SSF treatment were determined. Results showed that the fermentation treatment enhanced the release of functional molecules from CHMRs. The protocatechuic acid contents of Albizziae Cortex and Andrographis paniculata increased after fermenting with A. niger BCRC 32073, while presence of unknown compounds was observed for Eleutherococcus senticosus and Trichosanthes kirilowii Maxim. after fermentation. In addition, syringic acid, epicatechin gallate (ECG) and epicatechin gallate (ECG) were found for Lonicera japonica Thunb. and Trichosanthes kirilowii Maxim. after SSF with A. oryzae NCH 42. Moreover, the antimicrobial activity against some pathogenic bacteria increased for some fermented CHMRs, especially for those of Equisetum hiemale L., Bambusae Caulis in Taeniis., Lonicera japonica Thunb. and Trichosanthes kirilowii Maxim. These results revealed that solid state fermentation using either A. niger BCRC 32073 or A. oryzae NCH 42 increased antimicrobial activities and changed phenolic substance content of some CHMs, it therefore improves the utilization of CHMs.

並列關鍵字

Chinese herbal medicinal residues ； Solid state fermentation ； Antimicrobial activity ； Aspergillus niger ； Aspergillus oryzae