傳統與現代昆蟲鑑定技術的結合－以蘋果蠹蛾（鱗翅目：捲葉蛾科）為例

A combination of traditional and modern techniques for insect identification - using the codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), as an example

路光暉(Lu, K. H.) ； 張世忠(Chang, S. C.) ； 許昭元(Hsu, J. Y.) ； 楊正澤(Yang, J. T.)

《植物保護學會會刊》 45卷4期 (2003/12) Pp. 359-364

https://doi.org/10.6715/PPB.200312_45(4).0010

摘要

無資料

關鍵字

檢疫 ； 蘋果蠹蛾 ； 形態鑑定 ； 核酸鑑定

並列摘要

In this work, we combined traditional and modern techniques, i.e., morphological and species-specific DNA markers, respectively, to identify the codling moth, Cydia pomonella L. According to a published article, morphological characters, mainly the style of the proleg crochets and the number and distribution of setae, etc., were applied for identification of codling moth larvae. The rationale of sequence characterized amplified regions (SCARs) was adopted to develop DNA markers for identification of codling moths, and we obtained 5 pairs of species-specific PCR primers for this purpose. By applying these 2 methods, we successfully identified 2 codling moth specimens which were discovered in 2 batches of apples imported from the US in the course of quarantine inspections by the Kaohsiung Division of the Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ) on November 6 and 12, 2002, respectively.

並列關鍵字

quarantine ； codling moth ； Cydia pomonella ； morphological identification ； DNA identification