鹽分對五梨跤(Rhizophora stylosa)苗木生理反應之影響

Effects of Salinity on Physiological Responses of the Mangrove Rhizophora Stylosa Seedlings

范貴珠(Kuei-Chu Fan) ； 徐誠宏(Cheng-Hung Shiu)

《中華林學季刊》 42卷3期 (2009/09) Pp. 347-361

https://doi.org/10.30064/QJCF.200909.0003

摘要

本研究探討鹽度對五梨跤(Rhizophora stylosa)苗木之水分狀態、葉綠素濃度、葉及根部細胞三苯基氯化四唑(Triphenyl tetrazolium chloride, TTC)還原性、氯離子及脯胺酸(proline)濃度等生理反應之影響。胎生苗(viviparous seedling)以0.75、1.5、3.0% NaCl 及對照組(0%)等4 種鹽度處理60、120 及150天後，結果顯示0.75、1.5及3.0% NaCl 處理之葉部及莖含水率較高，對照組處理最低，惟苗木根部含水率則無明顯差異。各處理苗木成熟葉之相對含水率(RWC)並無顯著差異，大致維持在68.0-79.3%。而葉片之葉綠素a、b及總葉綠素濃度以0.75% NaCl處理最高，對照組最低，總葉綠素濃度僅1.21 mg g^(-1)。各處理苗木葉部之TTC還原性並無顯著差異，根部TTC細胞還原性則以0.75% NaCl處理之8.03 O.D. g^(-1)最高。不同鹽度處理60及150天後，葉部之氯離子濃度以3.0% NaCl處理較高，可達78.10 mg g^(-1)；根部之氯離子濃度則以0.75% NaCl處理者最高。不同鹽度處理150天後，苗木葉部脯氨酸濃度以0.75及1.5%NaCl處理者較高，分別為0.57及0.47 μ mole g^(-1)。以上結果顯示五梨跤苗木在低鹽分濃度環境下，較具正常的生理作用。

關鍵字

鹽度 ； 五梨跤 ； 水分狀態 ； 葉綠素濃度 ； 三苯基氯化四唑TTC還原性 ； 脯氨酸

並列摘要

This study examined the effects of salinity on physiological responses including water status, chlorophyll concentration, triphenyl tetrazolium chloride (TTC) reduction, Cl(superscript -) and proline concentrations of the mangrove Rhizophora stylosa seedlings. The viviparous seedlings were grown in various concentrations of salinity containing 0.75, 1.5, 3.0% NaCl and control (0%) for 60, 120, and 150 days. The results indicated that leaf and stem water content of seedlings were high for those cultivated in 0.75, 1.5 and 3.0% NaCl, and were lowest for those in 0% NaCl. However, root water content of seedlings was not significantly affected by salinity. The salinity level showed less effect on relative water content (RWC) of the fully expanded leaves, which was maintained at 68.0-79.3%. Chlorophylls a, b concentrations and the total amount of chlorophylls were highest for seedlings cultivated in 0.75% NaCl, and were the lowest for the 0% NaCl treatment, the total amount of chlorophylls was only 1.21 mg g^(-1). TTC reduction of leaf tissue was not significantly affected by salinity, though seedlings grown at 0.75% NaCl had the highest TTC reduction in root tissue (8.03 O.D. g^(-1)). After 60 and 150 days of treatment, leaf Cl(superscript -) concentration was high for seedlings cultivated in 3.0% NaCl, reaching 78.10 mg g^(-1). Moreover, seedlings grown at 0.75% NaCl showed the highest root Cl(superscript -) concentration. Leaf proline concentrations were high for seedlings cultivated in 0.75 and 1.5% NaCl at day 150, these values were 0.57 and 0.47 μ mole g^(-1), respectively. These results indicated that R. stylosa seedlings could have better physiological viability when grown in mild salinity.

並列關鍵字

Chlorophyll concentration ； Proline ； Rhizophora stylosa ； Salinity ； Triphenyl tetrazolium chloride TTC reduction ； Water status