臺灣豆馬勃屬新紀錄種與槲櫟實生苗之親合性探討

COMPATIBILITY OF A NEWLY RECORDED PISOLITHUS SPECIE WITH QUERCUS ALIENA SEEDLING

蔡滰桁(Ching-Heng Tsai) ； 張琮柏(Tsung-Po Chang) ； 周文能(Wen-Neng Chou) ； 文紀鑾(Chi-Luan Wen) ； 林瑞進(Lei-Chen Lin)

《中華林學季刊》 48卷3期 (2015/09) Pp. 221-230

摘要

殼斗科植物多數具有外生菌根；本研究的子實體採自於嘉義縣中埔鄉殼斗科植物林下，此子實體所分離純化的菌株編號為Cms01；利用玻片培養可發現其菌絲形態具有扣子體；利用分子生物技術分析，其ITS序列經比對結果與Pisolithus orientalis (NR119745)相似度最高；並探討其與槲櫟(Quercus aliena)之親合性，經接種3個月後，證實此菌株能與槲櫟共生；利用電子顯微鏡觀察，可於其根菌結合體發現具有明顯菌毯及哈替氏網等外生菌根之特徵。綜合上述結果，證實此次分離的菌株應屬於台灣新紀錄種P. orientalis，並且能和槲櫟共生形成外生菌根，此亦證實此菌株為外生菌根菌。

關鍵字

外生菌根 ； 外生菌根菌 ； 全合成 ； 豆馬勃屬 ； 槲櫟

並列摘要

The mycorrhizae of most Fagaceae species belong to ectomycorrhizae. In this study, the basidiocarps were collected from a Fagaceae spp. stand at Jhongpu area in Chiayi county. The no. Cms01 strain was isolated from the basidiocarp, it had found the clamp connection of mycelium by a slide culture, and the ITS serial alignments had high similarity with Pisolithus orientalis (NR119745) by molecular analysis. In aseptic synthesis experiment, this strain (Cms01) could form a mantle and a Hartig net in root associations with Quercus aliena by scanning electron microscope 3 months after inoculation. These results showed that this strain (Cms01) was a newly recorded species in the fungal flora of Taiwan, and it could form a typical ectomycorrhiza with Q. aliena seedling, and it also belonged to an ectomycorrhizal fungus.

並列關鍵字

Ectomycorrhizae ； Ectomycorrhizal fungus ； aseptic synthesis ； Pisolithus ； Quercus aliena